Vti1b antibody - 164 005

Vti1b is a SNARE protein involved in endosomal fusion events

Guinea pig polyclonal purified antibody

Cat. No.: 164 005

Amount: 50 µg

Price: $460.00

Cat. No. 164 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 206 from rat Vti1b (UniProt Id: P58200)
Reactivity	Reacts with: rat (P58200), mouse (O88384). Other species not tested yet.
Matching control protein/peptide	164-0P
Data sheet	164_005.pdf

References for Vti1b - 164 005

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 164 005 ICC; tested species: mouse

Cat. No.: 164 005

Amount: 50 µg

Price: $460.00

ICC

IHC

IHC-P

Background

Vti1b is a member of the SNARE family of proteins. It predominantly localizes to endosomal membranes, vesicles and tubules of the TGN. Vti1b is involved in the fusion of late endosomes and forms complexes with endobrevin, syntaxin 7 and syntaxin 8.

1 ) Murray RZ et al., J. Biol. Chem. (2005)
2 ) Hirst J et al., Mol. Biol. Cell (2004)
3 ) Atlashkin V et al., Mol. Cell. Biol. (2003)
4 ) Antonin W et al., J. Biol. Chem. (2002)
5 ) Kreykenbohm V et al., Eur. J. Cell Biol. (2002)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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