Shank1 antibody - 162 121AbRED

Shanks are multidomain proteins of the postsynaptic density

Mouse monoclonal purified antibody

Cat. No.: 162 121AbRED

Amount: 100 µg

Price: $470.00

Cat. No. 162 121AbRED	100 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with abberior STAR RED. abberior STAR^® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR^® dyes established fluorescence detection systems can be used. abberior STAR^® 635P: λ_ex 638 nm / λ_em 651 nm abberior STAR^® ORANGE: λ_ex 589 nm / λ_em 616 nm abberior STAR^® RED: λ_ex 638 nm / λ_em 655 nm This product or portions thereof is manufactured under license from abberior GmbH. abberior STAR is a trademark of abberior GmbH, Goettingen/Germany. Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: N/A Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	abberior STAR RED
Clone	329H10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank1 (UniProt Id: Q9WV48)
Remarks	ICC: This antibody conjugate is especially suitable for high resolution STED-microscopy.
Data sheet	162_121abred.pdf

Cat. No.: 162 121AbRED

Amount: 100 µg

Price: $470.00

ICC

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

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