Synaptic Systems - Caveolin1

Cat. No.: 161 011

Amount: 100 µg

Price: $420.00

WB

ICC

IHC

IHC-P

IHC-Fr

IHC-G

Background

Caveolae are distinct flask shaped invaginations which contain high concentrations of cholesterol and sphingolipids. These subcellular compartments can be found at the surface of many cell types. The primary structural proteins of caveolae are caveolins, a family comprising three members: caveolin1, caveolin2, and caveolin3. Caveolin1 exists in two isoforms, caveolin1α and caveolin1β, which differ in their N-terminal sequences. Caveolin1 is ubiquitously expressed in all cell types and is the predominant coat protein of endothelial caveolae. Caveolin1 is essential in multiple cellular processes, including membrane trafficking, cholesterol homeostasis, signal transduction, and cellular communication (1). Knockout studies have demonstrated that the absence of caveolin1 leads to a complete loss of caveolae structures in various cell types, underscoring its essential role in caveolae formation (2). Its dysregulation has been implicated in cardiovascular diseases, neurodegenerative disorders, metabolic diseases, and cancer (3). Caveolin1 plays a complex role in glioblastoma (GBM), the most aggressive primary brain tumor. Its function in GBM appears to be context-dependent, exhibiting both tumor-promoting and tumor-suppressing activities (4).

1 ) Williams TM et al., Genome Biol (2004)
2 ) Hnasko R et al., Mol Interv (2003)
3 ) Nwosu ZC et al., Mol Cancer (2016)
4 ) Moriconi C et al., Front Oncol (2021)

Other products for this target

Cat. No. 161 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 1000 gallery IHC-Fr: 1 : 500 (see remarks) gallery IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	SY-177E4
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to cytoplasatic domain of mouse Caveolin1 (UniProt Id: P49817)
Reactivity	Reacts with: mouse (P49817), rat (P41350), human (Q03135). Other species not tested yet.
Remarks	ICC: Methanol fixation is recommended. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6 or Tris-EDTA buffer pH 9.0) is required for immunohistochemical staining. IHC-Fr: Acetone, Formaldehyde, Methanol or Methanol-acetone (1:1 mix) fixation are recommended. IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH₂O, pH 4.2-4.4, according to Richter et al. 2017 or 9% glyoxal, 8% acetic acid, in ddH₂O, pH 4.2-4.4, according to Konno et al. 2023 are recommended.
Data sheet	161_011.pdf

Caveolin1 antibody - 161 011