Synaptic Systems - Caveolin1

Cat. No.: 161 002

Amount: 200 µl

Price: $360.00

WB

Background

Caveolae are distinct flask shaped invaginations which contain high concentrations of cholesterol and sphingolipids. These subcellular compartments can be found at the surface of many cell types. The primary structural proteins of caveolae are caveolins, a family comprising three members: caveolin1, caveolin2, and caveolin3. Caveolin1 exists in two isoforms, caveolin1α and caveolin1β, which differ in their N-terminal sequences. Caveolin1 is ubiquitously expressed in all cell types and is the predominant coat protein of endothelial caveolae. Caveolin1 is essential in multiple cellular processes, including membrane trafficking, cholesterol homeostasis, signal transduction, and cellular communication (1). Knockout studies have demonstrated that the absence of caveolin1 leads to a complete loss of caveolae structures in various cell types, underscoring its essential role in caveolae formation (2). Its dysregulation has been implicated in cardiovascular diseases, neurodegenerative disorders, metabolic diseases, and cancer (3). Caveolin1 plays a complex role in glioblastoma (GBM), the most aggressive primary brain tumor. Its function in GBM appears to be context-dependent, exhibiting both tumor-promoting and tumor-suppressing activities (4).

1 ) Williams TM et al., Genome Biol (2004)
2 ) Hnasko R et al., Mol Interv (2003)
3 ) Nwosu ZC et al., Mol Cancer (2016)
4 ) Moriconi C et al., Front Oncol (2021)

Protocols

WB

IP

Standard protocol

OTHER

Preabsorbtion

Other products for this target

Cat. No. 161 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not recommended IHC: not recommended IHC-P: not tested yet IHC-Fr: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 17 from rat Caveolin1 (UniProt Id: P41350)
Reactivity	Reacts with: human (Q03135), rat (P41350), mouse (P49817), dog, pig, cow, monkey. No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	161-0P
Data sheet	161_002.pdf

Caveolin1 antibody - 161 002