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Home > Products > 152 505

Ca2+ channel T-type alpha-1G antibody - 152 505

Guinea pig polyclonal purified antibody
Cat. No.: 152 505
Amount: 50 µg
Price: $460.00
Cat. No. 152 505 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1921 to 2254 from rat Ca2+ channel T-type α-1G (Cav3.1) (UniProt Id: O54898)
Reactivity Reacts with: rat (O54898), mouse (Q9WUT2).
Other species not tested yet.
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel T-type alpha-1G aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 152_505.pdf
Cat. No.: 152 505
Amount: 50 µg
Price: $460.00
WB
IHC
Background
Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1G isoform occurs in VGCCs of the T-type which belongs to the high voltage activated group (hva).
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