Ca2+ channel P/Q-type alpha-1A antibody - 152 211

Mouse monoclonal purified IgG

Cat. No.: 152 211

Amount: 100 µg

Price: $420.00

Cat. No. 152 211	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	23F11
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P54282)
Reactivity	Reacts with: rat (P54282), mouse (P97445). Other species not tested yet.
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. Less sensitive than the polyclonal antibodies (152 103, 152 205). ICC: 2% formaldehyde/PFA fixation is recommended. IHC-Fr: The following fixatives are possible: methanol, acetone, methanol-acetone, 4% formaldehyde/PFA
Data sheet	152_211.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 211

Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 211 WB; tested species: rat

Cat. No.: 152 211

Amount: 100 µg

Price: $420.00

ICC

IHC-Fr

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Wu LG et al., J. Neurosci. (1999)
2 ) Westenbroek RE et al., J. Neurosci. (1998)
3 ) Sakurai T et al., J. Cell Biol. (1996)
4 ) Westenbroek RE et al., J. Neurosci. (1995)
5 ) Sakurai T et al., J. Biol. Chem. (1995)
6 ) Starr TV et al., Proc. Natl. Acad. Sci. U.S.A. (1991)

Protocols

ICC

IHC-Fr

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