Gephyrin antibody - 147 011AF568 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors

Mouse monoclonal purified IgG

Cat. No.: 147 011AF568

Amount: 100 µg

Price: $470.00

Cat. No. 147 011AF568	100 µg purified IgG, lyophilized, fluorescence-labeled with AZdye 568. Fluorescence labeled antibodies conjugated to Alexa^® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye^® 568: λ_ex 568 nm / λ_em 665 nm (identical to Alexa^® 568) Alexa^® 647: λ_ex 650 nm / λ_em 665 nm This product or portions thereof is manufactured under license from Life Technologies Corporation. Alexa Fluor is a registered trademark of Life Technologies Corporation. Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A ICC: 1 : 500 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not recommended ELISA: N/A Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	AZdye 568
Clone	mAb7a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop	AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: human (Q9NQX3), mouse (Q8BUV3), rat (Q03555), goldfish, chicken, pig, zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270 K.O. validated
Data sheet	147_011af568.pdf

Cat. No.: 147 011AF568

Amount: 100 µg

Price: $470.00

ICC

Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

1 ) Hermann A et al., Biochem. Biophys. Res. Commun. (2001)
2 ) Kirsch J et al., Brain Res. (1993)
3 ) Kirsch J et al., Eur. J. Neurosci. (1993)

Protocols

ICC

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