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Glycine receptor alpha1 antibody - 146 118

An inhibitory receptor in the nervous system
Rabbit monoclonal recombinant IgG
Cat. No.: 146 118
Amount: 50 µg
Price: $415.00
Cat. No. 146 118 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not recommended
Clone RbmAb2b
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop AA 29 to 39 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415), rat (P07727), mouse (Q64018), pig, ape.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone mAb2b. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: To improve signal strength, antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is highly recommended.

Data sheet 146_118.pdf

References for Glycine receptor alpha1 - 146 118

Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease.
Wiessler AL, Hasenmüller AS, Fuhl I, Mille C, Cortes Campo O, Reinhard N, Schenk J, Heinze KG, Schaefer N, Specht CG, Villmann C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 442: . 146 118 ICC; tested species: mouse
Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix-A Model System to Study Disease Mechanisms.
Fischhaber N, Faber J, Bakirci E, Dalton PD, Budday S, Villmann C, Schaefer N
Advanced healthcare materials (2021) 1019: e2100830. 146 118 ICC; tested species: mouse
Cat. No.: 146 118
Amount: 50 µg
Price: $415.00
Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease.
Wiessler AL, Hasenmüller AS, Fuhl I, Mille C, Cortes Campo O, Reinhard N, Schenk J, Heinze KG, Schaefer N, Specht CG, Villmann C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 442: . 146 118 ICC; tested species: mouse
Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix-A Model System to Study Disease Mechanisms.
Fischhaber N, Faber J, Bakirci E, Dalton PD, Budday S, Villmann C, Schaefer N
Advanced healthcare materials (2021) 1019: e2100830. 146 118 ICC; tested species: mouse
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.