Bassoon antibody - 141 111AbRED K.O.

Bassoon is a large multi-domain protein of the presynaptic active zone

Mouse monoclonal purified IgG

Cat. No.: 141 111AbRED

Amount: 100 µg

Price: $465.00

Cat. No. 141 111AbRED	100 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR RED. abberior STAR^® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR^® dyes established fluorescence detection systems can be used. abberior STAR^® 635P: λ_ex 638 nm / λ_em 651 nm abberior STAR^® ORANGE: λ_ex 589 nm / λ_em 616 nm abberior STAR^® RED: λ_ex 638 nm / λ_em 655 nm This product or portions thereof is manufactured under license from abberior GmbH. abberior STAR is a trademark of abberior GmbH, Goettingen/Germany. Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	abberior STAR RED
Clone	179H11A2
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the central region of rat Bassoon (UniProt Id: O88778)
Reactivity	Reacts with: mouse (O88737), rat (O88778). Other species not tested yet.
Specificity	K.O. validated
Remarks	ICC: This antibody conjugate is especially suitable for high resolution STED microscopy.
Data sheet	141_111abred.pdf

Cat. No.: 141 111AbRED

Amount: 100 µg

Price: $465.00

ICC

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

ICC

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