VAChT antibody - 139 017AbOR

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles

Rat monoclonal purified IgG

Cat. No.: 139 017AbOR

Amount: 100 µg

Price: $470.00

Cat. No. 139 017AbOR	100 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR ORANGE. abberior STAR^® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR^® dyes established fluorescence detection systems can be used. abberior STAR^® 635P: λ_ex 638 nm / λ_em 651 nm abberior STAR^® ORANGE: λ_ex 589 nm / λ_em 616 nm abberior STAR^® RED: λ_ex 638 nm / λ_em 655 nm This product or portions thereof is manufactured under license from abberior GmbH. abberior STAR is a trademark of abberior GmbH, Goettingen/Germany. Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: N/A Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	abberior STAR ORANGE
Clone	15A11
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity	Reacts with: mouse (O35304), rat (Q62666). Other species not tested yet.
Remarks	ICC: This antibody conjugate is especially suitable for high resolution STED-microscopy.
Data sheet	139_017abor.pdf

Cat. No.: 139 017AbOR

Amount: 100 µg

Price: $470.00

ICC

Background

The vesicular acetylcholine transporter VAChT is an integral membrane protein with 12 putative trans-membrane domains. VAChT and choline acetyltransferase (ChAT) are encoded by genes organized in a single gene locus, and coregulation of the two genes has been reported several times.
VAChT translocates acetylcholine from the cytoplasm into synaptic vesicles where it stays until release. After release from the presynaptic nerve terminal acetylcholine is hydrolyzed by acetylcholine esterase. During Alzheimer´s disease acetylcholine is one of the first neurotransmitters to be reduced.

1 ) Roghani A et al., Brain Res. Mol. Brain Res. (2002)
2 ) Dolezal V et al., Brain Res. Bull. (2001)
3 ) Mallet J et al., J. Physiol. Paris (1998)
4 ) Li JY et al., Neurochem. Int. ()
5 ) Arvidsson U et al., J. Comp. Neurol. (1997)

Protocols

ICC

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