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VAChT antibody - 139 017

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles
Rat monoclonal purified IgG
Cat. No.: 139 017
Amount: 100 µg
Price: $415.00
Cat. No. 139 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: yes (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 gallery  
Clone 15A11
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: mouse (O35304), rat (Q62666).
Other species not tested yet.
Specificity Specific for VAChT
Matching control protein/peptide 139-1P
Remarks

WB: VAChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 139_017.pdf
Cat. No.: 139 017
Amount: 100 µg
Price: $415.00
Background
The vesicular acetylcholine transporter VAChT is an integral membrane protein with 12 putative trans-membrane domains. VAChT and choline acetyltransferase (ChAT) are encoded by genes organized in a single gene locus, and coregulation of the two genes has been reported several times.
VAChT translocates acetylcholine from the cytoplasm into synaptic vesicles where it stays until release. After release from the presynaptic nerve terminal acetylcholine is hydrolyzed by acetylcholine esterase. During Alzheimer´s disease acetylcholine is one of the first neurotransmitters to be reduced.