Cat. No.: 135 503
Amount: 50 µg
Price:
$380.00
|
|
|
| Cat. No. 135 503 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to AA 324 to 339 from rat VGLUT1 (UniProt Id: Q62634) |
| Reactivity |
Reacts with: human (Q9P2U7, Q9P2U8), rat (Q62634, Q9JI12), mouse (Q3TXX4, Q8BLE7), mammals, chicken, zebrafish, frog. Other species not tested yet. |
| Specificity | Epitope identical in VGLUT 1 and VGLUT 2, one mismatch in VGLUT 3. |
| Remarks |
This antibody is less powerful compared to those directed against the C-termini of VGLUTs but can be used for non-mammals like chicken and zebrafish.VGLUTs aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
| Data sheet | 135_503.pdf |
|
|
The vesicular glutamate transporter 1 , VGLUT 1 also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.