Cat. No.: 135 421C5
Amount: 100 µg
Price:
465.00 €
| Cat. No. 135 421C5 |
100 µg purified IgG, lyophilized, fluorescence-labeled with Sulfo-Cyanine 5. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
WB: N/A IP: N/A ICC: not recommended (see remarks) IHC: 1 : 200 up to 1 : 500 gallery IHC-P/FFPE: not tested yet |
| Label | Sulfo-Cyanine 5 |
| Clone | 95E11 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12) |
| Reactivity |
Reacts with: rat (Q9JI12), mouse (Q8BLE7). Other species not tested yet. |
| Specificity | Specific for VGLUT 2 |
| Remarks |
ICC: As a Sulfo-Cyanine 5 conjugate this antibody produces some unspecifc nuclear staining in cultured primary neurons. |
| Data sheet | 135_421c5.pdf |
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The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
