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Home > Products > 135 416

VGLUT2 antibody - 135 416

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Chicken polyclonal purified antibody
Cat. No.: 135 416
Amount: 50 µg
Price: $385.00
Cat. No. 135 416 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 5000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Matching control protein/peptide 135-4P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 135_416.pdf

References for VGLUT2 - 135 416

Behavioral decline in Shank3Δex4-22 mice during early adulthood parallels cerebellar granule cell glutamatergic synaptic changes.
Kshetri R, Beavers JO, Hyde R, Ewa R, Schwertman A, Porcayo S, Richardson BD
Molecular autism (2024) 151: 52. 135 416 IHC; tested species: mouse
Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.
Quillet R, Gutierrez-Mecinas M, Polgár E, Dickie AC, Boyle KA, Watanabe M, Todd AJ
Frontiers in molecular neuroscience (2023) 16: 1294994. 135 416 IHC; tested species: mouse
Glutamate delta 1 receptor regulates autophagy mechanisms and affects excitatory synapse maturation in the somatosensory cortex.
Gawande DY, S Narasimhan KK, Bhatt JM, Pavuluri R, Kesherwani V, Suryavanshi PS, Shelkar GP, Dravid SM
Pharmacological research (2022) 178: 106144. 135 416 IHC; tested species: mouse
Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 135 416 IHC; tested species: mouse
Local targets of T-stellate cells in the ventral cochlear nucleus.
Lin L, Campbell J, Oertel D, Smith PH
The Journal of comparative neurology (2022) : . 135 416 IHC; tested species: mouse
Antibodies Against the Gastrin-releasing Peptide Precursor Pro-Gastrin-releasing Peptide Reveal Its Expression in the Mouse Spinal Dorsal Horn.
Gutierrez-Mecinas M, Kókai É, Polgár E, Quillet R, Titterton HF, Weir GA, Watanabe M, Todd AJ
Neuroscience (2022) 510: 60-71. 135 416 IHC; tested species: mouse
EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Wang Y, Eddison M, Fleishman G, Weigert M, Xu S, Wang T, Rokicki K, Goina C, Henry FE, Lemire AL, Schmidt U, et al.
Cell (2021) : . 135 416 IHC; tested species: mouse
Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb.
Naik AS, Lin JM, Taroc EZM, Katreddi RR, Frias JA, Lemus AA, Sammons MA, Forni PE
Development (Cambridge, England) (2020) 1478: . 135 416 IHC; tested species: mouse
Optogenetic "low-theta" pacing of the septohippocampal circuit is sufficient for spatial goal finding and is influenced by behavioral state and cognitive demand.
Mouchati PR, Kloc ML, Holmes GL, White SL, Barry JM
Hippocampus (2020) 3011: 1167-1193. 135 416 IHC; tested species: rat
Circuit dissection of the role of somatostatin in itch and pain.
Huang J, Polgár E, Solinski HJ, Mishra SK, Tseng PY, Iwagaki N, Boyle KA, Dickie AC, Kriegbaum MC, Wildner H, Zeilhofer HU, et al.
Nature neuroscience (2018) : . 135 416 IHC; tested species: mouse
Cat. No.: 135 416
Amount: 50 µg
Price: $385.00
Behavioral decline in Shank3Δex4-22 mice during early adulthood parallels cerebellar granule cell glutamatergic synaptic changes.
Kshetri R, Beavers JO, Hyde R, Ewa R, Schwertman A, Porcayo S, Richardson BD
Molecular autism (2024) 151: 52. 135 416 IHC; tested species: mouse
Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.
Quillet R, Gutierrez-Mecinas M, Polgár E, Dickie AC, Boyle KA, Watanabe M, Todd AJ
Frontiers in molecular neuroscience (2023) 16: 1294994. 135 416 IHC; tested species: mouse
Glutamate delta 1 receptor regulates autophagy mechanisms and affects excitatory synapse maturation in the somatosensory cortex.
Gawande DY, S Narasimhan KK, Bhatt JM, Pavuluri R, Kesherwani V, Suryavanshi PS, Shelkar GP, Dravid SM
Pharmacological research (2022) 178: 106144. 135 416 IHC; tested species: mouse
Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 135 416 IHC; tested species: mouse
Local targets of T-stellate cells in the ventral cochlear nucleus.
Lin L, Campbell J, Oertel D, Smith PH
The Journal of comparative neurology (2022) : . 135 416 IHC; tested species: mouse
Antibodies Against the Gastrin-releasing Peptide Precursor Pro-Gastrin-releasing Peptide Reveal Its Expression in the Mouse Spinal Dorsal Horn.
Gutierrez-Mecinas M, Kókai É, Polgár E, Quillet R, Titterton HF, Weir GA, Watanabe M, Todd AJ
Neuroscience (2022) 510: 60-71. 135 416 IHC; tested species: mouse
EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Wang Y, Eddison M, Fleishman G, Weigert M, Xu S, Wang T, Rokicki K, Goina C, Henry FE, Lemire AL, Schmidt U, et al.
Cell (2021) : . 135 416 IHC; tested species: mouse
Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb.
Naik AS, Lin JM, Taroc EZM, Katreddi RR, Frias JA, Lemus AA, Sammons MA, Forni PE
Development (Cambridge, England) (2020) 1478: . 135 416 IHC; tested species: mouse
Optogenetic "low-theta" pacing of the septohippocampal circuit is sufficient for spatial goal finding and is influenced by behavioral state and cognitive demand.
Mouchati PR, Kloc ML, Holmes GL, White SL, Barry JM
Hippocampus (2020) 3011: 1167-1193. 135 416 IHC; tested species: rat
Circuit dissection of the role of somatostatin in itch and pain.
Huang J, Polgár E, Solinski HJ, Mishra SK, Tseng PY, Iwagaki N, Boyle KA, Dickie AC, Kriegbaum MC, Wildner H, Zeilhofer HU, et al.
Nature neuroscience (2018) : . 135 416 IHC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
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