Synaptic Systems

Cat. No.: 135 307

Amount: 200 µl

Price: $330.00

For the import of goat antibodies into the USA you need a special import permit (APHIS). This can be applied for at your USDA office. If you have any questions, please contact us.

Cat. No. 135 307	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 1000 up to 1 : 10000 gallery IHC: 1 : 300 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity	Reacts with: rat (Q62634), mouse (Q3TXX4). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	135-3P
Remarks	This antibody is highly recommended as a marker for glutamatergic nerve terminals. WB: VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. This antibody is highly recommended as marker for glutamatergic nerve terminals and gives excellent results in ICC.
Data sheet	135_307.pdf

References for VGLUT1 - 135 307

CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice.
Halperin D, Stavsky A, Kadir R, Drabkin M, Wormser O, Yogev Y, Dolgin V, Proskorovski-Ohayon R, Perez Y, Nudelman H, Stoler O, et al.
Nature communications (2021) 121: 6187. 135 307 ICC; tested species: mouse

Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation.
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, et al.
Communications biology (2021) 41: 666. 135 307 ICC; tested species: mouse

PSD-95 promotes the stabilization of young synaptic contacts.
Taft CE, Turrigiano GG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691633: 20130134. 135 307 ICC; tested species: rat

Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 135 307 IHC; tested species: mouse

Mesenchymal-Derived Extracellular Vesicles Enhance Microglia-mediated Synapse Remodeling after Cortical Injury in Rhesus Monkeys.
Zhou Y, Bhatt H, Mojica CA, Xin H, Pessina M, Rosene DL, Moore TL, Medalla M
Research square (2023) : . 135 307 IHC; tested species: monkey

Mesenchymal-derived extracellular vesicles enhance microglia-mediated synapse remodeling after cortical injury in aging Rhesus monkeys.
Zhou Y, Bhatt H, Mojica CA, Xin H, Pessina MA, Rosene DL, Moore TL, Medalla M
Journal of neuroinflammation (2023) 201: 201. 135 307 IHC; tested species: monkey

Visualizing Synaptic Multi-Protein Patterns of Neuronal Tissue With DNA-Assisted Single-Molecule Localization Microscopy.
Narayanasamy KK, Stojic A, Li Y, Sass S, Hesse MR, Deussner-Helfmann NS, Dietz MS, Kuner T, Klevanski M, Heilemann M
Frontiers in synaptic neuroscience (2021) 13: 671288. 135 307 IHC; tested species: rat

Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain.
Olthof BM, Gartside SE, Rees A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 135 307 IHC

The ZIP3 zinc transporter is localized to mossy fiber terminals and is required for kainate-induced degeneration of CA3 neurons.
Bogdanovic M, Asraf H, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 135 307 IHC-P; tested species: mouse

Cat. No.: 135 307

Amount: 200 µl

Price: $330.00

ICC

IHC

Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

1 ) Takamori S et al., Nature (2000)
2 ) Bellocchio EE et al., Science (2000)
3 ) Bellocchio EE et al., J. Neurosci. (1998)
4 ) Ni B et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

VGLUT1 antibody - 135 307 K.O.

References for VGLUT1 - 135 307