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Home > Products > 135 303C3

VGLUT1 antibody - 135 303C3 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 135 303C3
Amount: 50 µg
Price: $490.00
Cat. No. 135 303C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 10000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, sheep, dog.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-3P
Remarks

This antibody is highly recommended as markers for glutamatergic nerve terminals.
Data sheet 135_303c3.pdf

References for VGLUT1 - 135 303C3

ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells.
Park NI, Guilhamon P, Desai K, McAdam RF, Langille E, O'Connor M, Lan X, Whetstone H, Coutinho FJ, Vanner RJ, Ling E, et al.
Cell stem cell (2017) 212: 209-224.e7. 135 303C3 ICC; tested species: mouse
Cat. No.: 135 303C3
Amount: 50 µg
Price: $490.00
ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells.
Park NI, Guilhamon P, Desai K, McAdam RF, Langille E, O'Connor M, Lan X, Whetstone H, Coutinho FJ, Vanner RJ, Ling E, et al.
Cell stem cell (2017) 212: 209-224.e7. 135 303C3 ICC; tested species: mouse
ICC
IHC
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
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