Synaptic Systems

Cat. No.: 135 211

Amount: 100 µg

Price: $415.00

Cat. No. 135 211	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	57A8
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Reactivity	Reacts with: rat (Q7TSF2), mouse (Q8BFU8). Other species not tested yet.
Remarks	WB: Due to the low abundance of this protein in the brain, immunoblotting is difficult.
Data sheet	135_211.pdf

References for VGLUT3 - 135 211

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 135 211 WB, IP; tested species: mouse

Regenerated hair cells in the neonatal cochlea are innervated and the majority co-express markers of both inner and outer hair cells.
Heuermann ML, Matos S, Hamilton D, Cox BC
Frontiers in cellular neuroscience (2022) 16: 841864. 135 211 IHC; tested species: mouse

Early-life sevoflurane exposure impairs fear memory by suppressing extracellular signal-regulated kinase signaling in the bed nucleus of stria terminalis GABAergic neurons.
Zhao XP, Zhong F, Luo RY, Zhang YL, Luo C, Li H, Dai RP
Neuropharmacology (2021) 191: 108584. 135 211 IHC; tested species: mouse

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 135 211 IHC, EM; tested species: rat

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 211 UPTAKE; tested species: rat

Cat. No.: 135 211

Amount: 100 µg

Price: $415.00

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 135 211 IHC, EM; tested species: rat

IHC

IHC-P

Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.

1 ) Landry M et al., J. Comp. Neurol. (2004)
2 ) Haverkamp S et al., J. Comp. Neurol. (2004)
3 ) Harkany T et al., Eur. J. Neurosci. (2003)
4 ) Oliveira AL et al., Synapse (2003)
5 ) Fremeau RT et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
6 ) Schäfer MK et al., J. Biol. Chem. (2002)
7 ) Gras C et al., J. Neurosci. (2002)

Protocols

Standard protocol

IHC

IHC-P

Other products for this target

VGLUT3 antibody - 135 211

References for VGLUT3 - 135 211