VGLUT3 antibody - 135 208

VGluT3 is a synaptic vesicle glutamate transporter

Rabbit monoclonal recombinant IgG

Cat. No.: 135 208

Amount: 50 µg

Price: $415.00

Cat. No. 135 208	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb57A8
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Reactivity	Reacts with: rat (Q7TSF2), mouse (Q8BFU8). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 57A8. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	135_208.pdf

References for VGLUT3 - 135 208

Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 208 IHC; tested species: mouse

Cat. No.: 135 208

Amount: 50 µg

Price: $415.00

Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 208 IHC; tested species: mouse

IHC

IHC-P

Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.

1 ) Landry M et al., J. Comp. Neurol. (2004)
2 ) Haverkamp S et al., J. Comp. Neurol. (2004)
3 ) Harkany T et al., Eur. J. Neurosci. (2003)
4 ) Oliveira AL et al., Synapse (2003)
5 ) Fremeau RT et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
6 ) Schäfer MK et al., J. Biol. Chem. (2002)
7 ) Gras C et al., J. Neurosci. (2002)

Protocols

IHC

IHC-P

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