Synaptic Systems

Cat. No.: 135 204

Amount: 100 µl

Price: $370.00

Cat. No. 135 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse VGLUT3 (UniProt Id: Q8BFU8)
Reactivity	Reacts with: mouse (Q8BFU8), rat (Q7TSF2). Other species not tested yet.
Specificity	K.O. validated PubMed: 28559797
Matching control protein/peptide	135-2P
Remarks	WB: Due to the low abundance of this protein in the brain, immunoblotting is difficult.
Data sheet	135_204.pdf

References for VGLUT3 - 135 204

Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 204 WB, IHC; KO verified; tested species: mouse

Oxidative Stress Plays an Important Role in Glutamatergic Excitotoxicity-Induced Cochlear Synaptopathy: Implication for Therapeutic Molecules Screening.
Saidia AR, François F, Casas F, Mechaly I, Venteo S, Veechi JT, Ruel J, Puel JL, Wang J
Antioxidants (Basel, Switzerland) (2024) 132: . 135 204 ICC; tested species: mouse

Retinal ganglion cell-derived semaphorin 6A segregates starburst amacrine cell dendritic scaffolds to organize the inner retina.
James RE, Hamilton NR, Huffman LN, Neckles VN, Pasterkamp RJ, Goff LA, Kolodkin AL
Development (Cambridge, England) (2024) : . 135 204 IHC; tested species: mouse

Adaptation to photoperiod via dynamic neurotransmitter segregation.
Maddaloni G, Chang YJ, Senft RA, Dymecki SM
Nature (2024) 6328023: 147-156. 135 204 IHC; tested species: mouse

Early-Onset Hearing Loss in Mouse Models of Alzheimer's Disease and Increased DNA Damage in the Cochlea.
Park JH, Sahbaz BD, Pekhale K, Chu X, Okur MN, Grati M, Isgrig K, Chien W, Chrysostomou E, Sullivan L, Croteau DL, et al.
Aging Biology (2024) 1: . 135 204 IHC; tested species: mouse

Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere.
Fortin-Houde J, Henderson F, Dumas S, Ducharme G, Amilhon B
The Journal of comparative neurology (2023) 5317: 702-719. 135 204 IHC; tested species: mouse

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 135 204 IHC; tested species: mouse

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.
Wen L, Yang X, Wu Z, Fu S, Zhan Y, Chen Z, Bi D, Shen Y
Cell reports (2023) 424: 112349. 135 204 IHC; tested species: mouse

A disease-associated mutation in thyroid hormone receptor α1 causes hearing loss and sensory hair cell patterning defects in mice.
Affortit C, Blanc F, Nasr J, Ceccato JC, Markossian S, Guyot R, Puel JL, Flamant F, Wang J
Science signaling (2022) 15738: eabj4583. 135 204 IHC; tested species: mouse

Impulse Noise Induced Hidden Hearing Loss, Hair Cell Ciliary Changes and Oxidative Stress in Mice.
Gratias P, Nasr J, Affortit C, Ceccato JC, François F, Casas F, Pujol R, Pucheu S, Puel JL, Wang J
Antioxidants (Basel, Switzerland) (2021) 1012: . 135 204 IHC; tested species: mouse

Sucrose Consumption Alters Serotonin/Glutamate Co-localisation Within the Prefrontal Cortex and Hippocampus of Mice.
Beecher K, Wang J, Jacques A, Chaaya N, Chehrehasa F, Belmer A, Bartlett SE
Frontiers in molecular neuroscience (2021) 14: 678267. 135 204 IHC; tested species: mouse

A Single Cisterna Magna Injection of AAV Leads to Binaural Transduction in Mice.
Blanc F, Bemelmans AP, Affortit C, Joséphine C, Puel JL, Mondain M, Wang J
Frontiers in cell and developmental biology (2021) 9: 783504. 135 204 IHC; tested species: mouse

Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 135 204 IHC; tested species: mouse

Neurochemically and Hodologically Distinct Ascending VGLUT3 versus Serotonin Subsystems Comprise the r2-Pet1 Median Raphe.
Senft RA, Freret ME, Sturrock N, Dymecki SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4112: 2581-2600. 135 204 IHC; tested species: mouse

Otoferlin Is Required for Proper Synapse Maturation and for Maintenance of Inner and Outer Hair Cells in Mouse Models for DFNB9.
Stalmann U, Franke AJ, Al-Moyed H, Strenzke N, Reisinger E
Frontiers in cellular neuroscience (2021) 15: 677543. 135 204 IHC; tested species: mouse

Cabp2-Gene Therapy Restores Inner Hair Cell Calcium Currents and Improves Hearing in a DFNB93 Mouse Model.
Oestreicher D, Picher MM, Rankovic V, Moser T, Pangrsic T
Frontiers in molecular neuroscience (2021) 14: 689415. 135 204 IHC; tested species: mouse

VGLUT3-p.A211V variant fuses stereocilia bundles and elongates synaptic ribbons.
Joshi Y, Petit CP, Miot S, Guillet M, Sendin G, Bourien J, Wang J, Pujol R, Mestikawy SE, Puel JL, Nouvian R, et al.
The Journal of physiology (2021) : . 135 204 IHC; tested species: mouse

Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 135 204 IHC; tested species: mouse

A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons.
Okaty BW, Sturrock N, Escobedo Lozoya Y, Chang Y, Senft RA, Lyon KA, Alekseyenko OV, Dymecki SM
eLife (2020) 9: . 135 204 IHC; tested species: mouse

Perinatal interference with the serotonergic system affects VTA function in the adult via glutamate co-transmission.
Cunha C, Smiley JF, Chuhma N, Shah R, Bleiwas C, Menezes EC, Seal RP, Edwards RH, Rayport S, Ansorge MS, Castellanos FX, et al.
Molecular psychiatry (2020) : . 135 204 IHC; tested species: mouse

Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 135 204 IHC; tested species: mouse

Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae.
Jiang D, Burger CA, Casasent AK, Albrecht NE, Li F, Samuel MA
The Journal of comparative neurology (2019) : . 135 204 IHC; tested species: mouse

Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.
Sonntag M, Blosa M, Schmidt S, Reimann K, Blum K, Eckrich T, Seeger G, Hecker D, Schick B, Arendt T, Engel J, et al.
BMC biology (2018) 161: 99. 135 204 IHC; tested species: mouse

Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus.
Heise C, Schroeder JC, Schoen M, Halbedl S, Reim D, Woelfle S, Kreutz MR, Schmeisser MJ, Boeckers TM
Frontiers in cellular neuroscience (2016) 10: 106. 135 204 IHC

Mice deficient of glutamatergic signaling from intrinsically photosensitive retinal ganglion cells exhibit abnormal circadian photoentrainment.
Purrier N, Engeland WC, Kofuji P
PloS one (2014) 910: e111449. 135 204 IHC; tested species: mouse

Cat. No.: 135 204

Amount: 100 µl

Price: $370.00

Adaptation to photoperiod via dynamic neurotransmitter segregation.
Maddaloni G, Chang YJ, Senft RA, Dymecki SM
Nature (2024) 6328023: 147-156. 135 204 IHC; tested species: mouse

IHC

Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.

1 ) Landry M et al., J. Comp. Neurol. (2004)
2 ) Haverkamp S et al., J. Comp. Neurol. (2004)
3 ) Harkany T et al., Eur. J. Neurosci. (2003)
4 ) Oliveira AL et al., Synapse (2003)
5 ) Fremeau RT et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
6 ) Schäfer MK et al., J. Biol. Chem. (2002)
7 ) Gras C et al., J. Neurosci. (2002)

Protocols

IHC

OTHER

Preabsorbtion

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VGLUT3 antibody - 135 204 K.O.

References for VGLUT3 - 135 204