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VGLUT1 antibody - 135 011AbRED

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified antibody
Cat. No.: 135 011AbRED
Amount: 100 µg
Price: $470.00
Cat. No. 135 011AbRED 100 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with abberior STAR RED.

abberior STAR® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR® dyes established fluorescence detection systems can be used.

abberior STAR® 635P: λex 638 nm / λem 651 nm
abberior STAR® ORANGE: λex 589 nm / λem 616 nm
abberior STAR® RED: λex 638 nm / λem 655 nm
 

This product or portions thereof is manufactured under license from abberior GmbH.
abberior STAR is a trademark of abberior GmbH, Goettingen/Germany.
Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH.

Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
ICC: 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: N/A

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.

Label abberior STAR RED
Clone 68B7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT1 (UniProt Id: Q62634)
Remarks

ICC: This antibody conjugate is especially suitable for high resolution STED-microscopy.

Data sheet 135_011abred.pdf
Cat. No.: 135 011AbRED
Amount: 100 µg
Price: $470.00
Background

The vesicular glutamate transporter 1 VGLUT1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT2, VGLUT1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT1 and VGLUT2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.