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Home > Products > 131 008AbOR

VGAT antibody cytoplasmic domain - 131 008AbOR K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit monoclonal recombinant IgG
Cat. No.: 131 008AbOR
Amount: 50 µg
Price: $470.00
Cat. No. 131 008AbOR 50 µg purified recombinant IgG, lyophilized, fluorescence-labeled with abberior STAR ORANGE.

abberior STAR® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR® dyes established fluorescence detection systems can be used.

abberior STAR® 635P: λex 638 nm / λem 651 nm
abberior STAR® ORANGE: λex 589 nm / λem 616 nm
abberior STAR® RED: λex 638 nm / λem 655 nm
 

This product or portions thereof is manufactured under license from abberior GmbH.
abberior STAR is a trademark of abberior GmbH, Goettingen/Germany.
Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH.
Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label abberior STAR ORANGE
Clone Rb117G4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633), human (Q9H598).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: This antibody conjugate is especially suitable for high resolution STED-microscopy.
Data sheet 131_008abor.pdf
Cat. No.: 131 008AbOR
Amount: 50 µg
Price: $470.00
ICC
ICC
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Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
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