Neuroligin4 antibody - 129 403 K.O.

Neuroligins are postsynaptic cell adhesion molecules

Rabbit polyclonal purified antibody

Cat. No.: 129 403

Amount: 50 µg

Price: $375.00

Cat. No. 129 403	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 686 to 699 from mouse Neuroligin4 (UniProt Id: B0F2B4)
Reactivity	Reacts with: mouse (B0F2B4). No signal: zebrafish, rat. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	129-4P
Data sheet	129_403.pdf

References for Neuroligin4 - 129 403

Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations.
Xu ZX, Kim GH, Tan JW, Riso AE, Sun Y, Xu EY, Liao GY, Xu H, Lee SH, Do NY, Lee CH, et al.
Nature communications (2020) 111: 1797. 129 403 WB; tested species: mouse

Cat. No.: 129 403

Amount: 50 µg

Price: $375.00

Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and 4 have been implicated with a rare, heritable form of autism.

1 ) Hoon M et al., Proc. Natl. Acad. Sci. U.S.A. (2011)
2 ) Chubykin AA et al., J. Biol. Chem. (2005)

Protocols

OTHER

Preabsorbtion

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