Synaptic Systems - Neuroligin3

Cat. No.: 129 103

Amount: 50 µg

Price: $375.00

Cat. No. 129 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not recommended ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Recombinant protein corresponding to AA 710 to 824 from mouse Neuroligin3 (UniProt Id: Q8BYM5)
Reactivity	Reacts with: rat (Q62889), mouse (Q8BYM5). Other species not tested yet.
Specificity	Specific for neuroligin 3. Detects other neuroligins when heavily overexpressed. K.O. validated
Matching control protein/peptide	129-1P
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 129 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	129_103.pdf

References for Neuroligin3 - 129 103

Neurexin-2: An inhibitory neurexin that restricts excitatory synapse formation in the hippocampus.
Lin PY, Chen LY, Jiang M, Trotter JH, Seigneur E, Südhof TC
Science advances (2023) 91: eadd8856. 129 103 WB; tested species: mouse

Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action.
Paskus JD, Tian C, Fingleton E, Shen C, Chen X, Li Y, Myers SA, Badger JD, Bemben MA, Herring BE, Roche KW, et al.
Cell reports (2019) 2910: 2944-2952.e5. 129 103 WB; tested species: rat

Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization.
Liu A, Zhou Z, Dang R, Zhu Y, Qi J, He G, Leung C, Pak D, Jia Z, Xie W
The Journal of cell biology (2016) 2124: 449-63. 129 103 WB

Synaptic Contacts Enhance Cell-to-Cell Tau Pathology Propagation.
Calafate S, Buist A, Miskiewicz K, Vijayan V, Daneels G, de Strooper B, de Wit J, Verstreken P, Moechars D
Cell reports (2015) 118: 1176-83. 129 103 WB; KD verified; tested species: rat

Promoter-like sequences regulating transcriptional activity in neurexin and neuroligin genes.
Runkel F, Rohlmann A, Reissner C, Brand SM, Missler M
Journal of neurochemistry (2013) 1271: 36-47. 129 103 WB

Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion.
Suckow AT, Comoletti D, Waldrop MA, Mosedale M, Egodage S, Taylor P, Chessler SD
Endocrinology (2008) 14912: 6006-17. 129 103 WB

Cat. No.: 129 103

Amount: 50 µg

Price: $375.00

Background

Neuroligins form a family of post-synaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

1 ) Song JY et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
2 ) Missler M et al., J. Neurochem. (1998)
3 ) Ichtchenko K et al., J. Biol. Chem. (1996)
4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Neuroligin3 antibody - 129 103 K.O.

References for Neuroligin3 - 129 103