Synaptic Systems - Munc13-1

Cat. No.: 126 117

Amount: 100 µg

Price: $420.00

WB

ICC

IHC

Background

Munc13s are homologues of the C. elegans unc-13 gene product. Three brain-specific isoforms, Munc 13-1, -13-2, and -13-3, are expressed in rat, where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb, and hippocampus. Munc13-2 is mainly expressed in cortex and hippocampus, whereas Munc13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled-coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc13-2) has been described.
Munc13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

1 ) Sheu L et al., J. Biol. Chem. (2003)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Ohtsuka T et al., J. Cell Biol. (2002)
4 ) Ashery U et al., EMBO J. (2000)
5 ) Brose N et al., Curr. Opin. Neurobiol. (2000)
6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

WB

ICC

IHC

Other products for this target

Cat. No. 126 117	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-147D6
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the N-terminus of rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: mouse (Q4KUS2), rat (Q62768). Other species not tested yet.
Specificity	K.O. validated
Data sheet	126_117.pdf

Munc13-1 antibody - 126 117 K.O.