Synaptic Systems

Cat. No.: 124 103

Amount: 50 µg

Price: $455.00

WB

ICC

ExM

Background

PSD93 (postsynaptic density protein of 93 kDa, also called chapsin 110 and DLG 2) belongs to the PSD95 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. It is a component of postsynaptic densities in central synapses.
PSD93 is expressed in discrete neuronal populations as well as in specific non-neuronal cells. It exhibits complex molecular diversity attributable to tissue-specific alternative splicing. PSD93, like PSD95, binds to NMDA receptors and to the neuronal nitric oxide synthase (NOS).
PSD93 and PSD95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. PSD93, however, is unique among PSD95 family members in its expression in Purkinje neuron cell bodies and dendrites.

1 ) Fujita A et al., Biochem. Biophys. Res. Commun. (2000)
2 ) Gundelfinger ED et al., Naturwissenschaften (2000)
3 ) Brenman JE et al., J. Neurosci. (1996)
4 ) Kim E et al., Neuron (1996)

Protocols

WB

IP

Standard protocol

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 124 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes , but special protocol (see Valtschanoff et al. 2000) ICC: 1 : 100 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ExM: 1 : 50 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Synthetic peptide corresponding to AA 22 to 37 from rat PSD93 (UniProt Id: Q63622)
Reactivity	Reacts with: human (Q15700), rat (Q63622), mouse (Q91XM9), hamster. No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	124-1P
Remarks	ICC: Methanol fixation is recommended. ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	124_103.pdf

PSD93 antibody - 124 103 K.O.