Synaptic Systems

Cat. No.: 119 011AT488

Amount: 100 µg

Price: please inquire

ICC

Background

SV2s (Synaptic Vesicle Protein 2) are integral membrane glycoproteins present in synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis (1). There are three characterized isoforms, SV2 A, SV2 B and SV2 C that are similar in structure but show different expression patterns. SV2 A is expressed ubiquitously throughout the brain and plays a crucial role in modulating synaptic transmission by regulating the expression and trafficking of synaptotagmin, a key calcium sensor in neurotransmitter release (1).
SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A and is predominantly found in the cortex and hippocampus (2). SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb (3).
SV2 expression has also been observed in other non-neuronal organs. In kidney it localizes to podocytes and is essential for the integrity of the glomerular filtration barrier (4).

1 ) Rossi R et al., Front Neurosci (2022)
2 ) Bartholome O et al., Front Mol Neurosci (2017)
3 ) Stout KA et al., ACS Chem Neurosci (2019)
4 ) Fukusumi Y et al., Lab. Invest. (2015)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 119 011AT488	100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO^® 488. For many of the fluorescence labeled antibodies conjugated to ATTO^® dyes from ATTO-TEC the established fluorescence detection systems can be used. ATTO^® 488: λ_ex 500 nm / λ_em 520 nm ATTO^® 550: λ_ex 554 nm / λ_em 576 nm ATTO^® 565: λ_ex 564 nm / λ_em 590 nm ATTO^® 594: λ_ex 603 nm / λ_em 626 nm ATTO^® 647N: λ_ex 646 nm / λ_em 664 nm ATTO^®488, ATTO^®565, ATTO^®647N and ATTO^®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. ATTO^®488, ATTO^®565 and ATTO^®594 are also recommended for PALM and dSTORM high resolution microscopy. This product or portions thereof is manufactured under license from ATTO-TEC GmbH. ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany. Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	ATTO 488
Clone	17G10
Subtype	IgG3 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of human SV2 A (UniProt Id: Q7L0J3)
Reactivity	Reacts with: rat (Q02563), mouse (Q9JIS5). Other species not tested yet. Predicted to cross-react with human (Q7L0J3) due to high sequence homology.
Matching control protein/peptide	119-0P
Data sheet	119_011at488.pdf

SV2 A antibody - 119 011AT488