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SNAP25B antibody - 111 113 K.O.

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Rabbit polyclonal purified antibody
Cat. No.: 111 113
Amount: 50 µg
Price: $375.00
Cat. No. 111 113 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 58 to 72 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: rat (P60881-1), mouse (P60879-1).
Other species not tested yet.
Specificity Specific for SNAP 25B, no cross-reactivity to SNAP 25A. K.O. validated PubMed: 31024034
Data sheet 111_113.pdf

References for SNAP25B - 111 113

SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 113 WB; KO verified; tested species: mouse
Cat. No.: 111 113
Amount: 50 µg
Price: $375.00
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 113 WB; KO verified; tested species: mouse
Background

SNAP25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.