Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 110 162

Syntaxin16 antibody - 110 162

Syntaxin16 is a SNARE protein involved in trans-Golgi network trafficking
Rabbit polyclonal antiserum
Cat. No.: 110 162
Amount: 200 µl
Price: $355.00
Cat. No. 110 162 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not recommended
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: yes
IHC-P: 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 302 from rat Syntaxin16 (UniProt Id: A0A0G2K528)
Reactivity Reacts with: human (O14662), rat (A0A0G2K528), mouse (Q8BVI5), hamster, rabbit.
Other species not tested yet.
Specificity Recognizes all four isoforms syntaxin 16a, b, c, d.
Matching control protein/peptide 110-16P
Data sheet 110_162.pdf

References for Syntaxin16 - 110 162

Phosphorylation of the N-terminus of Syntaxin-16 controls interaction with mVps45 and GLUT4 trafficking in adipocytes.
Bremner SK, Berends R, Kaupisch A, Roccisana J, Sutherland C, Bryant NJ, Gould GW
PeerJ (2023) 11: e15630. 110 162 WB; tested species: human,rat
EHD2 regulates plasma membrane integrity and downstream insulin receptor signalling events.
Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, Bryant NJ, et al.
Molecular biology of the cell (2023) : mbcE23030078. 110 162 WB; tested species: mouse
Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle.
Livingstone R, Bryant NJ, Boyle JG, Petrie JR, Gould GW
Endocrinology, diabetes & metabolism (2022) 55: e361. 110 162 WB; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 162 WB; tested species: mouse
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 162 WB; tested species: human
Dynamic GLUT4 sorting through a syntaxin-6 compartment in muscle cells is derailed by insulin resistance-causing ceramide.
Foley KP, Klip A
Biology open (2014) 35: 314-25. 110 162 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 110 162 WB
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 162 WB, ICC; KD verified; tested species: rabbit
Endoproteolytic cleavage of TUG protein regulates GLUT4 glucose transporter translocation.
Bogan JS, Rubin BR, Yu C, Löffler MG, Orme CM, Belman JP, McNally LJ, Hao M, Cresswell JA
The Journal of biological chemistry (2012) 28728: 23932-47. 110 162 WB
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 162 WB
Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Sadakata T, Shinoda Y, Sekine Y, Saruta C, Itakura M, Takahashi M, Furuichi T
The Journal of biological chemistry (2010) 28549: 38710-9. 110 162 WB
Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 110 162 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 162 WB
The v-SNARE Vti1a regulates insulin-stimulated glucose transport and Acrp30 secretion in 3T3-L1 adipocytes.
Bose A, Guilherme A, Huang S, Hubbard AC, Lane CR, Soriano NA, Czech MP
The Journal of biological chemistry (2005) 28044: 36946-51. 110 162 WB
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 162 WB
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 110 162 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 110 162 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 162 WB, ICC; KD verified; tested species: rabbit
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 162 ICC
Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomes.
Nishimoto-Morita K, Shin HW, Mitsuhashi H, Kitamura M, Zhang Q, Johannes L, Nakayama K
The Journal of biological chemistry (2009) 28416: 10583-92. 110 162 ICC
Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 110 162 WB, ICC
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 110 162 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 110 162 IHC
Cat. No.: 110 162
Amount: 200 µl
Price: $355.00
Phosphorylation of the N-terminus of Syntaxin-16 controls interaction with mVps45 and GLUT4 trafficking in adipocytes.
Bremner SK, Berends R, Kaupisch A, Roccisana J, Sutherland C, Bryant NJ, Gould GW
PeerJ (2023) 11: e15630. 110 162 WB; tested species: human,rat
EHD2 regulates plasma membrane integrity and downstream insulin receptor signalling events.
Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, Bryant NJ, et al.
Molecular biology of the cell (2023) : mbcE23030078. 110 162 WB; tested species: mouse
Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle.
Livingstone R, Bryant NJ, Boyle JG, Petrie JR, Gould GW
Endocrinology, diabetes & metabolism (2022) 55: e361. 110 162 WB; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 162 WB; tested species: mouse
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 162 WB; tested species: human
Dynamic GLUT4 sorting through a syntaxin-6 compartment in muscle cells is derailed by insulin resistance-causing ceramide.
Foley KP, Klip A
Biology open (2014) 35: 314-25. 110 162 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 110 162 WB
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 162 WB, ICC; KD verified; tested species: rabbit
Endoproteolytic cleavage of TUG protein regulates GLUT4 glucose transporter translocation.
Bogan JS, Rubin BR, Yu C, Löffler MG, Orme CM, Belman JP, McNally LJ, Hao M, Cresswell JA
The Journal of biological chemistry (2012) 28728: 23932-47. 110 162 WB
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 162 WB
Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Sadakata T, Shinoda Y, Sekine Y, Saruta C, Itakura M, Takahashi M, Furuichi T
The Journal of biological chemistry (2010) 28549: 38710-9. 110 162 WB
Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 110 162 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 162 WB
The v-SNARE Vti1a regulates insulin-stimulated glucose transport and Acrp30 secretion in 3T3-L1 adipocytes.
Bose A, Guilherme A, Huang S, Hubbard AC, Lane CR, Soriano NA, Czech MP
The Journal of biological chemistry (2005) 28044: 36946-51. 110 162 WB
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 162 WB
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 110 162 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 110 162 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 162 WB, ICC; KD verified; tested species: rabbit
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 162 ICC
Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomes.
Nishimoto-Morita K, Shin HW, Mitsuhashi H, Kitamura M, Zhang Q, Johannes L, Nakayama K
The Journal of biological chemistry (2009) 28416: 10583-92. 110 162 ICC
Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.
Pérez-Victoria FJ, Bonifacino JS
Molecular and cellular biology (2009) 2919: 5251-63. 110 162 WB, ICC
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 110 162 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 162 WB, ICC, IHC; tested species: mouse,rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 110 162 IHC
WB
ICC
IHC-P
Background

Syntaxin 16, a member of the SNARE family of proteins, localizes to the Golgi stack. It has been shown to be involved in trans-Golgi network trafficking and to interact with VAMP 3, VAMP 4 and VAMP 8.
Four splice variants (syntaxin 16a, b, c, d) have been described, which may have different roles in intracellular trafficking. The splice variant c is the shortest and localizes to the cytoplasm.
Other products for this target