Synaptic Systems - Syntaxin12/13

Cat. No.: 110 133

Amount: 50 µg

Price: $455.00

Cat. No. 110 133	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 250 from rat Syntaxin12/13 (UniProt Id: G3V7P1)
Reactivity	Reacts with: human (Q86Y82), rat (G3V7P1), mouse (Q9ER00), hamster, chicken. Other species not tested yet.
Matching control protein/peptide	110-13P
Data sheet	110_133.pdf

References for Syntaxin12/13 - 110 133

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 133 WB; tested species: human

Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes.
Yap CC, Digilio L, McMahon LP, Garcia ADR, Winckler B
The Journal of cell biology (2018) : . 110 133 WB; tested species: rat

GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.
Chiu SL, Diering GH, Ye B, Takamiya K, Chen CM, Jiang Y, Niranjan T, Schwartz CE, Wang T, Huganir RL
Neuron (2017) 936: 1405-1419.e8. 110 133 WB; tested species: rat

Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors.
Yang H, Takagi H, Konishi Y, Ageta H, Ikegami K, Yao I, Sato S, Hatanaka K, Inokuchi K, Seog DH, Setou M, et al.
PloS one (2008) 37: e2809. 110 133 WB; tested species: mouse,rat

Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling.
Simoes S, Guo J, Buitrago L, Qureshi YH, Feng X, Kothiya M, Cortes E, Patel V, Kannan S, Kim YH, Chang KT, et al.
Cell reports (2021) 3713: 110182. 110 133 ICC; tested species: mouse

Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB
Histochemistry and cell biology (2015) 1442: 133-46. 110 133 ICC

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 110 133 IHC; tested species: mouse

Cat. No.: 110 133

Amount: 50 µg

Price: $455.00

ICC

IHC-P

Background

Syntaxin 12/13 are orthologues of the same gene whose product is a member of the SNARE family of proteins. It is related to syntaxins 1-4 but is localized predominantly to early endosomes of a wide variety of cells. Syntaxin 12/13 appears to be involved in the recycling of membrane receptors such as the transferrin receptors where it mediates the fusion of endosomal membranes.

1 ) Antonin W et al., EMBO J. (2000)
2 ) Jahn R et al., Annu. Rev. Biochem. (1999)
3 ) Advani RJ et al., J. Biol. Chem. (1998)
4 ) Prekeris R et al., J. Cell Biol. (1998)

Protocols

ICC

IHC-P

OTHER

Preabsorbtion

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Syntaxin12/13 antibody - 110 133

References for Syntaxin12/13 - 110 133