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Home > Products > 110 132

Syntaxin12/13 antibody - 110 132 K.D.

Syntaxin12/13 is a syntaxin family member functioning in the recycling of plasma membrane receptors
Rabbit polyclonal antiserum
Cat. No.: 110 132
Amount: 200 µl
Price: $355.00
Cat. No. 110 132 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 250 from rat Syntaxin12/13 (UniProt Id: G3V7P1)
Reactivity Reacts with: human (Q86Y82), rat (G3V7P1), mouse (Q9ER00), hamster, chicken.
Other species not tested yet.
Specificity K.D. validated PubMed: 28202687
Matching control protein/peptide 110-13P
Data sheet 110_132.pdf

References for Syntaxin12/13 - 110 132

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 132 WB, ICC; tested species: human
Salmonella SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes.
Singh PK, Kapoor A, Lomash RM, Kumar K, Kamerkar SC, Pucadyil TJ, Mukhopadhyay A
The Journal of cell biology (2018) : . 110 132 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 132 WB, ICC; KD verified; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 132 WB, IHC; tested species: mouse,rat
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 132 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 132 WB
Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes.
Vetrivel KS, Cheng H, Lin W, Sakurai T, Li T, Nukina N, Wong PC, Xu H, Thinakaran G
The Journal of biological chemistry (2004) 27943: 44945-54. 110 132 WB
Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells.
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G
Molecular biology of the cell (2004) 1512: 5399-407. 110 132 WB, ICC
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 132 WB
GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.
Chiu SL, Diering GH, Ye B, Takamiya K, Chen CM, Jiang Y, Niranjan T, Schwartz CE, Wang T, Huganir RL
Neuron (2017) 936: 1405-1419.e8. 110 132 IP; tested species: rat
Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling.
Simoes S, Guo J, Buitrago L, Qureshi YH, Feng X, Kothiya M, Cortes E, Patel V, Kannan S, Kim YH, Chang KT, et al.
Cell reports (2021) 3713: 110182. 110 132 ICC; tested species: mouse
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 132 WB, ICC; tested species: human
Salmonella SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes.
Singh PK, Kapoor A, Lomash RM, Kumar K, Kamerkar SC, Pucadyil TJ, Mukhopadhyay A
The Journal of cell biology (2018) : . 110 132 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 132 WB, ICC; KD verified; tested species: human
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB
Histochemistry and cell biology (2015) 1442: 133-46. 110 132 ICC
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 132 WB, ICC
Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.
Bonanomi D, Menegon A, Miccio A, Ferrari G, Corradi A, Kao HT, Benfenati F, Valtorta F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2532: 7299-308. 110 132 ICC
Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells.
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G
Molecular biology of the cell (2004) 1512: 5399-407. 110 132 WB, ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 132 WB, IHC; tested species: mouse,rat
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
Brandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R
Proceedings of the National Academy of Sciences of the United States of America (2006) 1038: 2701-6. 110 132
Cat. No.: 110 132
Amount: 200 µl
Price: $355.00
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 132 WB, ICC; tested species: human
Salmonella SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes.
Singh PK, Kapoor A, Lomash RM, Kumar K, Kamerkar SC, Pucadyil TJ, Mukhopadhyay A
The Journal of cell biology (2018) : . 110 132 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 132 WB, ICC; KD verified; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 132 WB, IHC; tested species: mouse,rat
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 132 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 132 WB
Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes.
Vetrivel KS, Cheng H, Lin W, Sakurai T, Li T, Nukina N, Wong PC, Xu H, Thinakaran G
The Journal of biological chemistry (2004) 27943: 44945-54. 110 132 WB
Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells.
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G
Molecular biology of the cell (2004) 1512: 5399-407. 110 132 WB, ICC
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 132 WB
GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.
Chiu SL, Diering GH, Ye B, Takamiya K, Chen CM, Jiang Y, Niranjan T, Schwartz CE, Wang T, Huganir RL
Neuron (2017) 936: 1405-1419.e8. 110 132 IP; tested species: rat
Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling.
Simoes S, Guo J, Buitrago L, Qureshi YH, Feng X, Kothiya M, Cortes E, Patel V, Kannan S, Kim YH, Chang KT, et al.
Cell reports (2021) 3713: 110182. 110 132 ICC; tested species: mouse
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 132 WB, ICC; tested species: human
Salmonella SipA mimics a cognate SNARE for host Syntaxin8 to promote fusion with early endosomes.
Singh PK, Kapoor A, Lomash RM, Kumar K, Kamerkar SC, Pucadyil TJ, Mukhopadhyay A
The Journal of cell biology (2018) : . 110 132 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 132 WB, ICC; KD verified; tested species: human
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB
Histochemistry and cell biology (2015) 1442: 133-46. 110 132 ICC
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
Steuble M, Diep TM, Schätzle P, Ludwig A, Tagaya M, Kunz B, Sonderegger P
Biology open (2012) 18: 761-74. 110 132 WB, ICC
Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.
Bonanomi D, Menegon A, Miccio A, Ferrari G, Corradi A, Kao HT, Benfenati F, Valtorta F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2532: 7299-308. 110 132 ICC
Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells.
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G
Molecular biology of the cell (2004) 1512: 5399-407. 110 132 WB, ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 132 WB, IHC; tested species: mouse,rat
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
Brandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R
Proceedings of the National Academy of Sciences of the United States of America (2006) 1038: 2701-6. 110 132
WB
ICC
IHC
IHC
IHC-P
Background
Syntaxin 12/13 are orthologues of the same gene whose product is a member of the SNARE family of proteins. It is related to syntaxins 1-4 but is localized predominantly to early endosomes of a wide variety of cells. Syntaxin 12/13 appears to be involved in the recycling of membrane receptors such as the transferrin receptors where it mediates the fusion of endosomal membranes.
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