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Home > Products > 110 131

Syntaxin12/13 antibody - 110 131

Syntaxin12/13 is a syntaxin family member functioning in the recycling of plasma membrane receptors
Mouse monoclonal purified IgG
Cat. No.: 110 131
Amount: 100 µg
Price: $415.00
Cat. No. 110 131 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 151.2
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 250 from rat Syntaxin12/13 (UniProt Id: G3V7P1)
Reactivity Reacts with: rat (G3V7P1).
No signal: zebrafish, mouse (Q9ER00).
Other species not tested yet.
Matching control protein/peptide 110-13P
Data sheet 110_131.pdf

References for Syntaxin12/13 - 110 131

Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 110 131 ICC; tested species: rat
Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation.
Widagdo J, Chai YJ, Ridder MC, Chau YQ, Johnson RC, Sah P, Huganir RL, Anggono V
Cell reports (2015) : . 110 131 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 131 ICC; tested species: rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 110 131 ICC
Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation.
Deane EC, Ilie AE, Sizdahkhani S, Das Gupta M, Orlowski J, McKinney RA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 332: 595-610. 110 131 ICC
Cat. No.: 110 131
Amount: 100 µg
Price: $415.00
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 110 131 ICC; tested species: rat
Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation.
Widagdo J, Chai YJ, Ridder MC, Chau YQ, Johnson RC, Sah P, Huganir RL, Anggono V
Cell reports (2015) : . 110 131 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 110 131 ICC; tested species: rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 110 131 ICC
Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation.
Deane EC, Ilie AE, Sizdahkhani S, Das Gupta M, Orlowski J, McKinney RA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 332: 595-610. 110 131 ICC
WB
ICC
Background
Syntaxin 12/13 are orthologues of the same gene whose product is a member of the SNARE family of proteins. It is related to syntaxins 1-4 but is localized predominantly to early endosomes of a wide variety of cells. Syntaxin 12/13 appears to be involved in the recycling of membrane receptors such as the transferrin receptors where it mediates the fusion of endosomal membranes.
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