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Home > Products > 110 083

Syntaxin8 antibody - 110 083

Syntaxin8 is a SNARE protein involved in the fusion of late endosomes
Rabbit polyclonal purified antibody
Cat. No.: 110 083
Amount: 50 µg
Price: $375.00
Cat. No. 110 083 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 215 from rat Syntaxin8 (UniProt Id: Q9Z2Q7)
Reactivity Reacts with: human (Q9UNK0), rat (Q9Z2Q7), mouse (O88983), hamster.
No signal: zebrafish.
Other species not tested yet.
Matching control protein/peptide 110-8P
Data sheet 110_083.pdf

References for Syntaxin8 - 110 083

Dysregulation of the AP2M1 phosphorylation cycle by LRRK2 impairs endocytosis and leads to dopaminergic neurodegeneration.
Liu Q, Bautista-Gomez J, Higgins DA, Yu J, Xiong Y
Science signaling (2021) 14693: . 110 083 WB; tested species: mouse
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 083 WB, ICC; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 083 WB; tested species: mouse
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 083 WB, ICC
Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6.
Madan R, Rastogi R, Parashuraman S, Mukhopadhyay A
The Journal of biological chemistry (2012) 2878: 5574-87. 110 083 WB
Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages.
Offenhäuser C, Lei N, Roy S, Collins BM, Stow JL, Murray RZ
Traffic (Copenhagen, Denmark) (2011) 126: 762-73. 110 083 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 083 WB
Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL
The Journal of biological chemistry (2005) 28011: 10478-83. 110 083 WB, IP
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 083 WB, ICC
Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL
The Journal of biological chemistry (2005) 28011: 10478-83. 110 083 WB, IP
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 083 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 083 ICC; tested species: human
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 083 WB, ICC
A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels.
Flowerdew SE, Burgoyne RD
The Biochemical journal (2009) 4183: 529-40. 110 083 ICC
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 083 WB, ICC
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 110 083 IHC; tested species: mouse
Cat. No.: 110 083
Amount: 50 µg
Price: $375.00
Dysregulation of the AP2M1 phosphorylation cycle by LRRK2 impairs endocytosis and leads to dopaminergic neurodegeneration.
Liu Q, Bautista-Gomez J, Higgins DA, Yu J, Xiong Y
Science signaling (2021) 14693: . 110 083 WB; tested species: mouse
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 083 WB, ICC; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 083 WB; tested species: mouse
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 083 WB, ICC
Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6.
Madan R, Rastogi R, Parashuraman S, Mukhopadhyay A
The Journal of biological chemistry (2012) 2878: 5574-87. 110 083 WB
Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages.
Offenhäuser C, Lei N, Roy S, Collins BM, Stow JL, Murray RZ
Traffic (Copenhagen, Denmark) (2011) 126: 762-73. 110 083 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 110 083 WB
Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL
The Journal of biological chemistry (2005) 28011: 10478-83. 110 083 WB, IP
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 083 WB, ICC
Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL
The Journal of biological chemistry (2005) 28011: 10478-83. 110 083 WB, IP
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 083 WB, ICC; tested species: human
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 083 ICC; tested species: human
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 083 WB, ICC
A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels.
Flowerdew SE, Burgoyne RD
The Biochemical journal (2009) 4183: 529-40. 110 083 ICC
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Nakamura N, Fukuda H, Kato A, Hirose S
Molecular biology of the cell (2005) 164: 1696-710. 110 083 WB, ICC
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 110 083 IHC; tested species: mouse
WB
ICC
Background

Syntaxin 8, a member of the SNARE family of proteins, is functionally related to the C-terminus of SNAP 25. Syntaxin 8 is localized to endosomal membranes of a wide variety of cells and is involved in the fusion of late endosomes and lysosomes. In endosomal membranes, syntaxin 8 forms complexes with endobrevin, syntaxin 7 and vti1b.
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