Syntaxin7 antibody - 110 073

Syntaxin7 is a SNARE protein involved in endosomal and lysosomal fusion reactions

Rabbit polyclonal purified antibody

Cat. No.: 110 073

Amount: 50 µg

Price: $455.00

Cat. No. 110 073	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 236 from rat Syntaxin7 (UniProt Id: O70257)
Reactivity	Reacts with: human (O15400), rat (O70257), mouse (O70439). Other species not tested yet.
Matching control protein/peptide	110-7P
Data sheet	110_073.pdf

References for Syntaxin7 - 110 073

Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 110 073 WB; tested species: rat

Cat. No.: 110 073

Amount: 50 µg

Price: $455.00

Background

Syntaxin 7, a member of the SNARE family of proteins, is related to syntaxins 1-4 but localized to endosomal membranes of a wide variety of cells.
Syntaxin 7 is involved in the fusion of late endosomes and lysosomes. In endosomal membranes, it forms complexes with endobrevin, syntaxin 8 and vti1b.

1 ) Jahn R et al., Annu. Rev. Biochem. (1999)
2 ) Prekeris R et al., Mol. Biol. Cell (1999)
3 ) Advani RJ et al., J. Biol. Chem. (1998)

Protocols

OTHER

Preabsorbtion

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