Synaptic Systems - Syntaxin5

Cat. No.: 110 053BT

Amount: 50 µg

Price: $480.00

ICC

Background

Syntaxin 5, a member of the SNARE family of proteins, is functionally related to the yeast protein Sed5p. Two syntaxin 5 isoforms (35 and 42 kDa) have been described which are generated from the same mRNA by alternative translation initiation. The longer 42 kDa variant contains an N-terminal extension carrying a putative type II ER-retrieval signal.
Syntaxin 5 forms a SNARE complex with membrin, GOSR 1, rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
Recently syntaxin 5 has been shown to be involved in the processing and accumulation of β-APP in neuronal cells.

1 ) Suga K et al., J. Neurochem. (2005)
2 ) Tai G et al., Mol. Biol. Cell (2004)
3 ) Williams AL et al., Mol. Biol. Cell (2004)
4 ) Xu Y et al., Mol. Biol. Cell (2002)
5 ) Zhang T et al., J. Biol. Chem. (2001)
6 ) Rabouille C et al., Cell (1998)
7 ) Rowe T et al., Science (1998)
8 ) Hui N et al., Mol. Biol. Cell (1997)
9 ) Ravichandran V et al., J. Mol. Neurosci. (1997)
10 ) Dascher C et al., J. Biol. Chem. (1996)

Protocols

WB

IP

Standard protocol

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 110 053BT	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 IP: yes ICC: 1 : 100 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Immunogen	Recombinant protein corresponding to AA 1 to 279 from mouse Syntaxin5 (UniProt Id: Q8K1E0)
Reactivity	Reacts with: human (Q13190), rat (Q08851), mouse (Q8K1E0), hamster. Other species not tested yet.
Matching control protein/peptide	110-5P
Remarks	This antibody detects the 35 kDa and 42 kDa variants of syntaxin 5.
Data sheet	110_053bt.pdf

Syntaxin5 antibody - 110 053BT