Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 110 053

Syntaxin5 antibody - 110 053 K.D.

Syntaxin5 is a SNARE protein involved in ER-Golgi transport
Rabbit polyclonal purified antibody
Cat. No.: 110 053
Amount: 50 µg
Price: $375.00
Cat. No. 110 053 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 279 from mouse Syntaxin5 (UniProt Id: Q8K1E0)
Reactivity Reacts with: human (Q13190), rat (Q08851), mouse (Q8K1E0), hamster, monkey.
Other species not tested yet.
Specificity K.D. validated PubMed: 31356625
Matching control protein/peptide 110-5P
Remarks

This antibody detects the 35 kDa and 42 kDa variants of syntaxin 5.
Data sheet 110_053.pdf

References for Syntaxin5 - 110 053

Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.
Tetenborg S, Ariakia F, Martinez-Soler E, Shihabeddin E, Lazart IC, Miller AC, O'Brien J
Cellular and molecular life sciences : CMLS (2024) 811: 431. 110 053 WB, ICC; KD verified; tested species: human
Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites.
Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
Communications biology (2023) 61: 1008. 110 053 WB, ICC; KD verified; tested species: mouse
Sec22b is a critical and nonredundant regulator of plasma cell maintenance.
Bonaud A, Gargowitsch L, Gilbert SM, Rajan E, Canales-Herrerias P, Stockholm D, Rahman NF, Collins MO, Taskiran H, Hill DL, Alloatti A, et al.
Proceedings of the National Academy of Sciences of the United States of America (2023) 1202: e2213056120. 110 053 WB; tested species: mouse
ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
Hirata T, Yang J, Tomida S, Tokoro Y, Kinoshita T, Fujita M, Kizuka Y
The Journal of biological chemistry (2022) : 102444. 110 053 WB; tested species: human
Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes.
Asseck LY, Mehlhorn DG, Monroy JR, Ricardi MM, Breuninger H, Wallmeroth N, Berendzen KW, Nowrousian M, Xing S, Schwappach B, Bayer M, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1181: . 110 053 WB
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.
Yue X, Qian Y, Zhu L, Gim B, Bao M, Jia J, Jing S, Wang Y, Tan C, Bottanelli F, Ziltener P, et al.
BMC biology (2021) 191: 194. 110 053 WB; tested species: human
Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.
Yue X, Tiwari N, Zhu L, Ngo HDT, Lim JM, Gim B, Jing S, Wang Y, Qian Y, Lee I
Communications biology (2021) 41: 1370. 110 053 WB; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 053 WB, ICC; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 053 WB; tested species: mouse
Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Sadakata T, Shinoda Y, Sekine Y, Saruta C, Itakura M, Takahashi M, Furuichi T
The Journal of biological chemistry (2010) 28549: 38710-9. 110 053 WB
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
Boström P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Borén J, Olofsson SO, et al.
Nature cell biology (2007) 911: 1286-93. 110 053 WB, EM
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.
Tetenborg S, Ariakia F, Martinez-Soler E, Shihabeddin E, Lazart IC, Miller AC, O'Brien J
Cellular and molecular life sciences : CMLS (2024) 811: 431. 110 053 WB, ICC; KD verified; tested species: human
Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport.
Kim N, Kim TH, Kim C, Lee JE, Kang MG, Shin S, Jung M, Kim JS, Mun JY, Rhee HW, Park SY, et al.
Developmental cell (2023) 5819: 1950-1966.e8. 110 053 ICC; tested species: human
Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites.
Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
Communications biology (2023) 61: 1008. 110 053 WB, ICC; KD verified; tested species: mouse
Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.
Arango Duque G, Dion R, Matte C, Fabié A, Descoteaux J, Stäger S, Descoteaux A
Journal of immunology (Baltimore, Md. : 1950) (2021) 2079: 2297-2309. 110 053 ICC; tested species: mouse
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 053 WB, ICC; tested species: human
The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole.
Arango Duque G, Jardim A, Gagnon É, Fukuda M, Descoteaux A
PLoS pathogens (2019) 157: e1007982. 110 053 ICC; KD verified; tested species: mouse
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 053 ICC; tested species: human
Chemical Modulation of Endocytic Sorting Augments Adeno-associated Viral Transduction.
Berry GE, Asokan A
The Journal of biological chemistry (2016) 2912: 939-47. 110 053 ICC
SMAP2 regulates retrograde transport from recycling endosomes to the Golgi.
Matsudaira T, Uchida Y, Tanabe K, Kon S, Watanabe T, Taguchi T, Arai H
PloS one (2013) 87: e69145. 110 053 ICC; tested species: monkey
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 053 ICC
Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis.
Lee S, Uchida Y, Emoto K, Umeda M, Kuge O, Taguchi T, Arai H
Genes to cells : devoted to molecular & cellular mechanisms (2012) 178: 728-36. 110 053 ICC
Storage vesicles in neurons are related to Golgi complex alterations in mucopolysaccharidosis IIIB.
Vitry S, Bruyère J, Hocquemiller M, Bigou S, Ausseil J, Colle MA, Prévost MC, Heard JM
The American journal of pathology (2010) 1776: 2984-99. 110 053 ICC; tested species: mouse
Three homologous ArfGAPs participate in coat protein I-mediated transport.
Saitoh A, Shin HW, Yamada A, Waguri S, Nakayama K
The Journal of biological chemistry (2009) 28420: 13948-57. 110 053 ICC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 053 DNA-PAINT; tested species: rat
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
Boström P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Borén J, Olofsson SO, et al.
Nature cell biology (2007) 911: 1286-93. 110 053 WB, EM
Cat. No.: 110 053
Amount: 50 µg
Price: $375.00
Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.
Tetenborg S, Ariakia F, Martinez-Soler E, Shihabeddin E, Lazart IC, Miller AC, O'Brien J
Cellular and molecular life sciences : CMLS (2024) 811: 431. 110 053 WB, ICC; KD verified; tested species: human
Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites.
Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
Communications biology (2023) 61: 1008. 110 053 WB, ICC; KD verified; tested species: mouse
Sec22b is a critical and nonredundant regulator of plasma cell maintenance.
Bonaud A, Gargowitsch L, Gilbert SM, Rajan E, Canales-Herrerias P, Stockholm D, Rahman NF, Collins MO, Taskiran H, Hill DL, Alloatti A, et al.
Proceedings of the National Academy of Sciences of the United States of America (2023) 1202: e2213056120. 110 053 WB; tested species: mouse
ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
Hirata T, Yang J, Tomida S, Tokoro Y, Kinoshita T, Fujita M, Kizuka Y
The Journal of biological chemistry (2022) : 102444. 110 053 WB; tested species: human
Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes.
Asseck LY, Mehlhorn DG, Monroy JR, Ricardi MM, Breuninger H, Wallmeroth N, Berendzen KW, Nowrousian M, Xing S, Schwappach B, Bayer M, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1181: . 110 053 WB
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.
Yue X, Qian Y, Zhu L, Gim B, Bao M, Jia J, Jing S, Wang Y, Tan C, Bottanelli F, Ziltener P, et al.
BMC biology (2021) 191: 194. 110 053 WB; tested species: human
Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.
Yue X, Tiwari N, Zhu L, Ngo HDT, Lim JM, Gim B, Jing S, Wang Y, Qian Y, Lee I
Communications biology (2021) 41: 1370. 110 053 WB; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 053 WB, ICC; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 053 WB; tested species: mouse
Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Sadakata T, Shinoda Y, Sekine Y, Saruta C, Itakura M, Takahashi M, Furuichi T
The Journal of biological chemistry (2010) 28549: 38710-9. 110 053 WB
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
Boström P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Borén J, Olofsson SO, et al.
Nature cell biology (2007) 911: 1286-93. 110 053 WB, EM
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.
Tetenborg S, Ariakia F, Martinez-Soler E, Shihabeddin E, Lazart IC, Miller AC, O'Brien J
Cellular and molecular life sciences : CMLS (2024) 811: 431. 110 053 WB, ICC; KD verified; tested species: human
Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport.
Kim N, Kim TH, Kim C, Lee JE, Kang MG, Shin S, Jung M, Kim JS, Mun JY, Rhee HW, Park SY, et al.
Developmental cell (2023) 5819: 1950-1966.e8. 110 053 ICC; tested species: human
Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites.
Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
Communications biology (2023) 61: 1008. 110 053 WB, ICC; KD verified; tested species: mouse
Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.
Arango Duque G, Dion R, Matte C, Fabié A, Descoteaux J, Stäger S, Descoteaux A
Journal of immunology (Baltimore, Md. : 1950) (2021) 2079: 2297-2309. 110 053 ICC; tested species: mouse
Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 110 053 WB, IP, ICC; KD verified; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 110 053 WB, ICC; tested species: human
The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole.
Arango Duque G, Jardim A, Gagnon É, Fukuda M, Descoteaux A
PLoS pathogens (2019) 157: e1007982. 110 053 ICC; KD verified; tested species: mouse
Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 110 053 ICC; tested species: human
Chemical Modulation of Endocytic Sorting Augments Adeno-associated Viral Transduction.
Berry GE, Asokan A
The Journal of biological chemistry (2016) 2912: 939-47. 110 053 ICC
SMAP2 regulates retrograde transport from recycling endosomes to the Golgi.
Matsudaira T, Uchida Y, Tanabe K, Kon S, Watanabe T, Taguchi T, Arai H
PloS one (2013) 87: e69145. 110 053 ICC; tested species: monkey
SNARE protein expression and localization in human cytotoxic T lymphocytes.
Pattu V, Qu B, Schwarz EC, Strauss B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M
European journal of immunology (2012) 422: 470-5. 110 053 ICC
Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis.
Lee S, Uchida Y, Emoto K, Umeda M, Kuge O, Taguchi T, Arai H
Genes to cells : devoted to molecular & cellular mechanisms (2012) 178: 728-36. 110 053 ICC
Storage vesicles in neurons are related to Golgi complex alterations in mucopolysaccharidosis IIIB.
Vitry S, Bruyère J, Hocquemiller M, Bigou S, Ausseil J, Colle MA, Prévost MC, Heard JM
The American journal of pathology (2010) 1776: 2984-99. 110 053 ICC; tested species: mouse
Three homologous ArfGAPs participate in coat protein I-mediated transport.
Saitoh A, Shin HW, Yamada A, Waguri S, Nakayama K
The Journal of biological chemistry (2009) 28420: 13948-57. 110 053 ICC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 053 DNA-PAINT; tested species: rat
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
Boström P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Borén J, Olofsson SO, et al.
Nature cell biology (2007) 911: 1286-93. 110 053 WB, EM
WB
ICC
IHC
Background

Syntaxin 5, a member of the SNARE family of proteins, is functionally related to the yeast protein Sed5p. Two syntaxin 5 isoforms (35 and 42 kDa) have been described which are generated from the same mRNA by alternative translation initiation. The longer 42 kDa variant contains an N-terminal extension carrying a putative type II ER-retrieval signal.
Syntaxin 5 forms a SNARE complex with membrin, GOSR 1, rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
Recently syntaxin 5 has been shown to be involved in the processing and accumulation of β-APP in neuronal cells.
Other products for this target