Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 110 032

Syntaxin3 antibody - 110 032

A syntaxin family member involved in membrane transport to the plasma membrane
Rabbit polyclonal antiserum
Cat. No.: 110 032
Amount: 200 µl
Price: $355.00
Cat. No. 110 032 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 gallery  
IHC: 1 : 2500
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 260 from rat Syntaxin3 (UniProt Id: Q08849)
Reactivity Reacts with: human (Q13277), rat (Q08849), mouse (Q64704), hamster, pig, zebrafish.
Other species not tested yet.
Matching control protein/peptide 110-3P
Remarks

WB: The affinity purified antibody (cat. no. 110 033) gives stronger signals in Western blots.
Data sheet 110_032.pdf

References for Syntaxin3 - 110 032

The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 032 WB; tested species: mouse
Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.
Black HL, Livingstone R, Mastick CC, Al Tobi M, Taylor H, Geiser A, Stirrat L, Kioumourtzoglou D, Petrie JR, Boyle JG, Bryant NJ, et al.
Journal of cell science (2021) : . 110 032 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 032 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 110 032 WB; tested species: mouse
SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting.
Zulliger R, Conley SM, Mwoyosvi ML, Stuck MW, Azadi S, Naash MI
PloS one (2015) 109: e0138508. 110 032 WB, IP
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 110 032 WB
Tumor protein D52 controls trafficking of an apical endolysosomal secretory pathway in pancreatic acinar cells.
Messenger SW, Thomas DD, Falkowski MA, Byrne JA, Gorelick FS, Groblewski GE
American journal of physiology. Gastrointestinal and liver physiology (2013) 3056: G439-52. 110 032 WB; tested species: rat
How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 032 WB; tested species: pig
A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2.
Wang CC, Ng CP, Shi H, Liew HC, Guo K, Zeng Q, Hong W
Molecular and cellular biology (2010) 301: 333-43. 110 032 WB
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion.
Antonin W, Wagner M, Riedel D, Brose N, Jahn R
Molecular and cellular biology (2002) 225: 1545-54. 110 032 WB; tested species: mouse
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 032 WB; tested species: mouse
SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting.
Zulliger R, Conley SM, Mwoyosvi ML, Stuck MW, Azadi S, Naash MI
PloS one (2015) 109: e0138508. 110 032 WB, IP
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
Buggia-Prévot V, Fernandez CG, Riordan S, Vetrivel KS, Roseman J, Waters J, Bindokas VP, Vassar R, Thinakaran G
Molecular neurodegeneration (2014) 9: 1. 110 032 ICC
Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.
Kravtsov DV, Caputo C, Collaco A, Hoekstra N, Egan ME, Mooseker MS, Ameen NA
Traffic (Copenhagen, Denmark) (2012) 138: 1072-82. 110 032 IHC; tested species: mouse
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
Cat. No.: 110 032
Amount: 200 µl
Price: $355.00
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 032 WB; tested species: mouse
Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.
Black HL, Livingstone R, Mastick CC, Al Tobi M, Taylor H, Geiser A, Stirrat L, Kioumourtzoglou D, Petrie JR, Boyle JG, Bryant NJ, et al.
Journal of cell science (2021) : . 110 032 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 032 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 110 032 WB; tested species: mouse
SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting.
Zulliger R, Conley SM, Mwoyosvi ML, Stuck MW, Azadi S, Naash MI
PloS one (2015) 109: e0138508. 110 032 WB, IP
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 110 032 WB
Tumor protein D52 controls trafficking of an apical endolysosomal secretory pathway in pancreatic acinar cells.
Messenger SW, Thomas DD, Falkowski MA, Byrne JA, Gorelick FS, Groblewski GE
American journal of physiology. Gastrointestinal and liver physiology (2013) 3056: G439-52. 110 032 WB; tested species: rat
How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 032 WB; tested species: pig
A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2.
Wang CC, Ng CP, Shi H, Liew HC, Guo K, Zeng Q, Hong W
Molecular and cellular biology (2010) 301: 333-43. 110 032 WB
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion.
Antonin W, Wagner M, Riedel D, Brose N, Jahn R
Molecular and cellular biology (2002) 225: 1545-54. 110 032 WB; tested species: mouse
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 032 WB; tested species: mouse
SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting.
Zulliger R, Conley SM, Mwoyosvi ML, Stuck MW, Azadi S, Naash MI
PloS one (2015) 109: e0138508. 110 032 WB, IP
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
Buggia-Prévot V, Fernandez CG, Riordan S, Vetrivel KS, Roseman J, Waters J, Bindokas VP, Vassar R, Thinakaran G
Molecular neurodegeneration (2014) 9: 1. 110 032 ICC
Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.
Kravtsov DV, Caputo C, Collaco A, Hoekstra N, Egan ME, Mooseker MS, Ameen NA
Traffic (Copenhagen, Denmark) (2012) 138: 1072-82. 110 032 IHC; tested species: mouse
Sunday driver interacts with two distinct classes of axonal organelles.
Abe N, Almenar-Queralt A, Lillo C, Shen Z, Lozach J, Briggs SP, Williams DS, Goldstein LS, Cavalli V
The Journal of biological chemistry (2009) 28450: 34628-39. 110 032 WB, IP, IHC
ICC
Background

Syntaxin 3, a member of the SNARE family of proteins, is related to syntaxin 1. While often co-expressed with syntaxins 2 and 4 within the same cell type, its membrane localization is usually different, for instance in epithelial cells or in the exocrine pancreas.
Like syntaxins 1, 2 and 4 it appears to be involved in the fusion of transport vesicles with the plasma membrane.
Other products for this target