Synaptic Systems - Syntaxin2

Cat. No.: 110 022

Amount: 200 µl

Price: $355.00

Cat. No. 110 022	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 IHC: yes IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 265 from rat Syntaxin2 (UniProt Id: P50279)
Reactivity	Reacts with: human (P32856), rat (P50279), mouse (Q00262), hamster, pig, zebrafish. Other species not tested yet.
Matching control protein/peptide	110-2P
Data sheet	110_022.pdf

References for Syntaxin2 - 110 022

Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.
Black HL, Livingstone R, Mastick CC, Al Tobi M, Taylor H, Geiser A, Stirrat L, Kioumourtzoglou D, Petrie JR, Boyle JG, Bryant NJ, et al.
Journal of cell science (2021) : . 110 022 WB; tested species: mouse

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 022 WB; tested species: mouse

Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 110 022 WB; tested species: mouse

Tumor protein D52 controls trafficking of an apical endolysosomal secretory pathway in pancreatic acinar cells.
Messenger SW, Thomas DD, Falkowski MA, Byrne JA, Gorelick FS, Groblewski GE
American journal of physiology. Gastrointestinal and liver physiology (2013) 3056: G439-52. 110 022 WB; tested species: rat

Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.
Arasaki K, Roy CR
Traffic (Copenhagen, Denmark) (2010) 115: 587-600. 110 022 WB, ICC; tested species: human

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 022 WB, IP; tested species: pig

Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 110 022 WB; tested species: mouse

Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 110 022 WB, ICC; tested species: rat

A molecular basis underlying differences in the toxicity of botulinum serotypes A and E.
Bajohrs M, Rickman C, Binz T, Davletov B
EMBO reports (2004) 511: 1090-5. 110 022 ICC, WB; tested species: rat

Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion.
Antonin W, Wagner M, Riedel D, Brose N, Jahn R
Molecular and cellular biology (2002) 225: 1545-54. 110 022 WB; tested species: mouse

SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes.
Li X, Low SH, Miura M, Weimbs T
American journal of physiology. Renal physiology (2002) 2835: F1111-22. 110 022 WB, IHC; tested species: rat

Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 022 WB; tested species: mouse

Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens.
Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS
Current biology : CB (2014) 2414: 1565-1572. 110 022 ICC

Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.
de Andrade GB, Kunzelman L, Merrill MM, Fuerst PG
Molecular vision (2014) 20: 1422-33. 110 022 IHC

Distribution of plasma membrane-associated syntaxins 1 through 4 indicates distinct trafficking functions in the synaptic layers of the mouse retina.
Sherry DM, Mitchell R, Standifer KM, du Plessis B
BMC neuroscience (2006) 7: 54. 110 022 IHC; tested species: mouse

Cat. No.: 110 022

Amount: 200 µl

Price: $355.00

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 022 WB; tested species: mouse

Background

Syntaxin 2, also referred to as Epimorphin, a member of the SNARE family of proteins, is related to syntaxin 1. Like syntaxin 4 it is predominantly localized to the plasma membrane of a wide variety of cells.
Similar to syntaxins 1, 3 and 4, it appears to be involved in the fusion of transport vesicles with the plasma membrane.

1 ) Jahn R et al., Annu. Rev. Biochem. (1999)
2 ) Bennett MK et al., Cell (1993)
3 ) Hirai Y et al., Cell (1992)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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Syntaxin2 antibody - 110 022

References for Syntaxin2 - 110 022