Synaptotagmin7 antibody - 105 711

Synaptotagmin7 is a Ca²⁺ dependent regulator of exocytosis

Mouse monoclonal purified IgG

Cat. No.: 105 711

Amount: 100 µg

Price: $430.00

Cat. No. 105 711	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-208D9
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein comprising the cytoplasmic domain of rat Synaptotagmin7 (UniProt Id: Q62747)
Reactivity	Reacts with: rat (Q62747), mouse (Q9R0N7). Other species not tested yet.
Matching control protein/peptide	105-71P
Remarks	WB: Cat. no. 105 173 is recommended for this application.
Data sheet	105_711.pdf

Cat. No.: 105 711

Amount: 100 µg

Price: $430.00

ICC

IHC

Background

Synaptotagmin7 is a proposed regulator of Ca²⁺ dependent exocytosis like neurotransmitter release. It occurs in several splicing variants which are expressed in a developmentally regulated pattern in brain. The distinct roles for the alternative splicing isoforms have not yet been determined.
Synaptotagmin7 shows Ca²⁺ dependent oligomerization via its own C2 domains leading to the formation of large linear structures which reside at the fusion site of vesicles and plasma membrane. These oligomers may be involved in the modulation of Ca²⁺ dependent exocytosis by opening or dilating fusion pores.

1 ) Fukuda M et al., Biochem. J. (2002)
2 ) Fukuda M et al., J. Biol. Chem. (2002)
3 ) Sugita S et al., Neuron (2001)
4 ) Fukuda M et al., J. Biol. Chem. (2001)
5 ) Caler EV et al., J. Exp. Med. (2001)
6 ) Fukuda M et al., J. Biol. Chem. (2000)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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