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Home > Products > 105 311C3

Synaptotagmin1 antibody luminal domain - 105 311C3

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 311C3
Amount: 100 µg
Price: $465.00
Cat. No. 105 311C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_311c3.pdf

References for Synaptotagmin1 - 105 311C3

Synaptic disruption and CREB-regulated transcription are restored by K+ channel blockers in ALS.
Catanese A, Rajkumar S, Sommer D, Freisem D, Wirth A, Aly A, Massa-López D, Olivieri A, Torelli F, Ioannidis V, Lipecka J, et al.
EMBO molecular medicine (2021) 137: e13131. 105 311C3 ICC; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 105 311C3 ICC
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 105 311C3 UPTAKE; tested species: mouse
Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation.
Anni D, Weiss EM, Guhathakurta D, Akdas YE, Klueva J, Zeitler S, Andres-Alonso M, Huth T, Fejtova A
Cellular and molecular life sciences : CMLS (2021) : . 105 311C3 UPTAKE; tested species: rat
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 105 311C3 UPTAKE
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 105 311C3 UPTAKE
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 105 311C3 UPTAKE; tested species: rat
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
Piccoli G, Condliffe SB, Bauer M, Giesert F, Boldt K, De Astis S, Meixner A, Sarioglu H, Vogt-Weisenhorn DM, Wurst W, Gloeckner CJ, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 316: 2225-37. 105 311C3 UPTAKE
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 105 311C3 UPTAKE; tested species: rat
Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
Rizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R
Traffic (Copenhagen, Denmark) (2006) 79: 1163-76. 105 311C3 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311C3
Cat. No.: 105 311C3
Amount: 100 µg
Price: $465.00
Synaptic disruption and CREB-regulated transcription are restored by K+ channel blockers in ALS.
Catanese A, Rajkumar S, Sommer D, Freisem D, Wirth A, Aly A, Massa-López D, Olivieri A, Torelli F, Ioannidis V, Lipecka J, et al.
EMBO molecular medicine (2021) 137: e13131. 105 311C3 ICC; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 105 311C3 ICC
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 105 311C3 UPTAKE; tested species: mouse
Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation.
Anni D, Weiss EM, Guhathakurta D, Akdas YE, Klueva J, Zeitler S, Andres-Alonso M, Huth T, Fejtova A
Cellular and molecular life sciences : CMLS (2021) : . 105 311C3 UPTAKE; tested species: rat
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 105 311C3 UPTAKE
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 105 311C3 UPTAKE
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 105 311C3 UPTAKE; tested species: rat
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
Piccoli G, Condliffe SB, Bauer M, Giesert F, Boldt K, De Astis S, Meixner A, Sarioglu H, Vogt-Weisenhorn DM, Wurst W, Gloeckner CJ, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 316: 2225-37. 105 311C3 UPTAKE
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 105 311C3 UPTAKE; tested species: rat
Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
Rizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R
Traffic (Copenhagen, Denmark) (2006) 79: 1163-76. 105 311C3 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311C3
ICC
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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