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Home > Products > 105 308Ab635P

Synaptotagmin1 antibody luminal domain - 105 308Ab635P

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit monoclonal recombinant IgG
Cat. No.: 105 308Ab635P
Amount: 50 µg
Price: $465.00
Cat. No. 105 308Ab635P 50 µg purified recombinant IgG, lyophilized, fluorescence-labeled with abberior STAR 635P.

abberior STAR® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR® dyes established fluorescence detection systems can be used.

abberior STAR® 635P: λex 638 nm / λem 651 nm
abberior STAR® ORANGE: λex 589 nm / λem 616 nm
abberior STAR® RED: λex 638 nm / λem 655 nm
 

This product or portions thereof is manufactured under license from abberior GmbH.
abberior STAR is a trademark of abberior GmbH, Goettingen/Germany.
Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH.

 Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label abberior STAR 635P
Clone Rb604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096).
Other species not tested yet.
Remarks

The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 604.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody has been expressed in mammalian cells.
ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_308ab635p.pdf
Cat. No.: 105 308Ab635P
Amount: 50 µg
Price: $465.00
ICC
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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