Synaptotagmin1 antibody luminal domain - 105 103C5

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Rabbit polyclonal purified antibody

Cat. No.: 105 103C5

Amount: 50 µg

Price: $485.00

Cat. No. 105 103C5	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 5. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet
Label	Sulfo-Cyanine 5
Immunogen	Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity	Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Matching control protein/peptide	105-10P
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_103c5.pdf

References for Synaptotagmin1 - 105 103C5

Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes.
Ivanova D, Dobson KL, Gajbhiye A, Davenport EC, Hacker D, Ultanir SK, Trost M, Cousin MA
Science advances (2021) 718: . 105 103C5 ICC; tested species: mouse

Cat. No.: 105 103C5

Amount: 50 µg

Price: $485.00

ICC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M, Annual review of neuroscience (1998) 21: 75-95.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Südhof TC, Nature (1995) 3756533: 645-53.
4 ) Geppert M, Cell (1994) 794: 717-27.
5 ) Brose N, Science (New York, N.Y.) (1992) 2565059: 1021-5.

Protocols

ICC

OTHER

Preabsorbtion

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