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Synaptobrevin2 (VAMP2) antibody - 104 318 K.O.

Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion
Guinea pig monoclonal recombinant IgG
Cat. No.: 104 318
Amount: 50 µg
Price: $420.00
Cat. No. 104 318 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P: 1 : 400 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Clone Gp69.1
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 104-2P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents.
The antibody has been expressed in mammalian cells.

Important: Although the epitope is still present in the Botox B cleavage product (aa 1 - 76), it is not recognized, probably due to conformational masking of the epitope.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

Data sheet 104_318.pdf

References for Synaptobrevin2 - 104 318

VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 104 318 ICC; tested species: rat
Catecholaminergic dysfunction drives postural and locomotor deficits in a mouse model of spinal muscular atrophy.
Pagiazitis JG, Delestrée N, Sowoidnich L, Sivakumar N, Simon CM, Chatzisotiriou A, Albani M, Mentis GZ
Cell reports (2025) 441: 115147. 104 318 IHC; tested species: mouse
Cat. No.: 104 318
Amount: 50 µg
Price: $420.00
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 104 318 ICC; tested species: rat
Catecholaminergic dysfunction drives postural and locomotor deficits in a mouse model of spinal muscular atrophy.
Pagiazitis JG, Delestrée N, Sowoidnich L, Sivakumar N, Simon CM, Chatzisotiriou A, Albani M, Mentis GZ
Cell reports (2025) 441: 115147. 104 318 IHC; tested species: mouse
Background

Synaptobrevins, also known as vesicle-associated membrane proteins (VAMPs), are predominantly expressed in the nervous system and are classified within the brevin subfamily of the SNARE (Soluble NSF Attachment Protein Receptor) protein superfamily. Brevins are small integral transmembrane proteins characterized by a central SNARE motif, an N-terminal cytoplasmic domain, and a C-terminal transmembrane domain. As crucial components of the SNARE machinery, these proteins play an essential role in vesicular transport and membrane fusion processes within cells (1, 2, 3).
In addition to synaptobrevins, the brevin family includes other tissue-specific members such as cellubrevin (VAMP3), myobrevin (VAMP5), and endobrevin (VAMP8), which are expressed in various non-neuronal tissues (4, 5, 6). These isoforms exhibit distinct spatial expression profiles, suggesting specialized functions beyond the nervous system.
Two Synaptobrevin isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their regional distribution within the brain, indicating isoform-specific roles in neuroexocytosis (7).
Synaptobrevin1 (VAMP1) is supposed to be essential for the maintenance of nerve impulse transmission in neuromuscular synapses. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevin2 (VAMP2) is more abundant and widely distributed in the brain and has been shown to be mainly involved in the assembly of effective SNARE complexes, Ca2+-dependent SV exocytosis, and fast endocytosis in hippocampal synapses (8). It is also expressed in spinal cord dorsal horn neurons and implicated in inflammatory pain sensitization (9).
Synaptobrevins are target molecules for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule and thereby disrupt neurotransmitter release (10).