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Home > Products > 104 303

Endobrevin antibody - 104 303 K.O.

Endobrevin also known as Vamp8 is an endosomal fusion protein, member of the R-SNARE family
Rabbit polyclonal purified antibody
Cat. No.: 104 303
Amount: 50 µg
Price: $455.00
Cat. No. 104 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: yes
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 75 from rat Endobrevin (UniProt Id: Q9WUF4)
Reactivity Reacts with: human (Q9BV40), rat (Q9WUF4), mouse (O70404).
Other species not tested yet.
Specificity K.O. validated PubMed: 25138214
Data sheet 104_303.pdf

References for Endobrevin - 104 303

The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Schoppmeyer R, van Steen ACI, Kempers L, Timmerman AL, Nolte MA, Hombrink P, van Buul JD
Cell reports (2022) 383: 110243. 104 303 WB; tested species: human
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 104 303 IP, WB; tested species: human,mouse
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 104 303 WB
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 104 303 IP, WB; tested species: human,mouse
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, González Mancha N, Ter Beest M, van den Bogaart G
eLife (2017) 6: . 104 303 ICC; tested species: human
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Cat. No.: 104 303
Amount: 50 µg
Price: $455.00
The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Schoppmeyer R, van Steen ACI, Kempers L, Timmerman AL, Nolte MA, Hombrink P, van Buul JD
Cell reports (2022) 383: 110243. 104 303 WB; tested species: human
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 104 303 IP, WB; tested species: human,mouse
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 104 303 WB
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 104 303 IP, WB; tested species: human,mouse
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, González Mancha N, Ter Beest M, van den Bogaart G
eLife (2017) 6: . 104 303 ICC; tested species: human
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
WB
ICC
Background

Endobrevin/VAMP8, a member of the SNARE family of proteins, is a relative of synaptobrevin that is involved in the fusion of early and late endosomes. Endobrevin is expressed in most mammalian cells but appears to be absent from neurons. The protein is predominantly localized to early and late endosomal membranes but is also found on other membranes and organelles involved in endocytic membrane traffic. In the fusion of late endosomes it forms SNARE complexes with syntaxin 7, syntaxin 8 and vti1b.
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