Synaptic Systems - Endobrevin

Cat. No.: 104 302

Amount: 200 µl

Price: $355.00

Cat. No. 104 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: yes IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 75 from rat Endobrevin (UniProt Id: Q9WUF4)
Reactivity	Reacts with: human (Q9BV40), rat (Q9WUF4), mouse (O70404). Other species not tested yet.
Specificity	K.D. validated PubMed: 28202687
Data sheet	104_302.pdf

References for Endobrevin - 104 302

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 104 302 WB; tested species: mouse

The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.
West ZE, Aitcheson SM, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2021) 2211: 368-376. 104 302 WB; KD verified; tested species: mouse

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 104 302 WB; tested species: rat

Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 104 302 WB; tested species: human

Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes.
Morris S, Geoghegan ND, Sadler JBA, Koester AM, Black HL, Laub M, Miller L, Heffernan L, Simpson JC, Mastick CC, Cooper J, et al.
PeerJ (2020) 8: e8751. 104 302 WB; KD verified; tested species: mouse

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 104 302 WB; tested species: mouse

Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 104 302 WB; KD verified; tested species: mouse

Platelet-specific deletion of SNAP23 ablates granule secretion, substantially inhibiting arterial and venous thrombosis in mice.
Williams CM, Li Y, Brown E, Poole AW
Blood advances (2018) 224: 3627-3636. 104 302 WB; tested species: mouse

Oxidized phagosomal NOX2 complex is replenished from lysosomes.
Dingjan I, Linders PT, van den Bekerom L, Baranov MV, Halder P, Ter Beest M, van den Bogaart G
Journal of cell science (2017) 1307: 1285-1298. 104 302 WB, ICC; KD verified; tested species: human

VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release.
Dingjan I, Paardekooper LM, Verboogen DRJ, von Mollard GF, Ter Beest M, van den Bogaart G
European journal of cell biology (2017) 967: 705-714. 104 302 WB, ICC; KD verified; tested species: human

A proteomic approach to identify endosomal cargoes controlling cancer invasiveness.
Diaz-Vera J, Palmer S, Hernandez-Fernaud JR, Dornier E, Mitchell LE, Macpherson I, Edwards J, Zanivan S, Norman JC
Journal of cell science (2017) 1304: 697-711. 104 302 WB, IP, ICC, IHC-P

Leishmania major Promastigotes Evade LC3-Associated Phagocytosis through the Action of GP63.
Matte C, Casgrain PA, Séguin O, Moradin N, Hong WJ, Descoteaux A
PLoS pathogens (2016) 126: e1005690. 104 302 WB, ICC

Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses.
He J, Johnson JL, Monfregola J, Ramadass M, Pestonjamasp K, Napolitano G, Zhang J, Catz SD
Molecular biology of the cell (2016) 273: 572-87. 104 302 WB, ICC; tested species: mouse

Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 104 302 WB

SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles.
Kubo K, Kobayashi M, Nozaki S, Yagi C, Hatsuzawa K, Katoh Y, Shin HW, Takahashi S, Nakayama K
Biology open (2015) 47: 910-20. 104 302 WB

PICALM modulates autophagy activity and tau accumulation.
Moreau K, Fleming A, Imarisio S, Lopez Ramirez A, Mercer JL, Jimenez-Sanchez M, Bento CF, Puri C, Zavodszky E, Siddiqi F, Lavau CP, et al.
Nature communications (2014) 5: 4998. 104 302 WB; KD verified; tested species: human

Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.
Tajika Y, Takahashi M, Khairani AF, Ueno H, Murakami T, Yorifuji H
Histochemistry and cell biology (2014) 1414: 441-54. 104 302 WB, ICC

Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages.
Offenhäuser C, Lei N, Roy S, Collins BM, Stow JL, Murray RZ
Traffic (Copenhagen, Denmark) (2011) 126: 762-73. 104 302 WB

Vesicle-associated membrane protein (VAMP) cleavage by a new metalloprotease from the Brazilian scorpion Tityus serrulatus.
Fletcher PL, Fletcher MD, Weninger K, Anderson TE, Martin BM
The Journal of biological chemistry (2010) 28510: 7405-16. 104 302 WB

A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2.
Wang CC, Ng CP, Shi H, Liew HC, Guo K, Zeng Q, Hong W
Molecular and cellular biology (2010) 301: 333-43. 104 302 WB, IHC

Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms.
Puri N, Roche PA
Proceedings of the National Academy of Sciences of the United States of America (2008) 1057: 2580-5. 104 302 WB; tested species: mouse

Vesicle-associated membrane protein-8/endobrevin negatively regulates phagocytosis of bacteria in dendritic cells.
Ho YH, Cai DT, Wang CC, Huang D, Wong SH
Journal of immunology (Baltimore, Md. : 1950) (2008) 1805: 3148-57. 104 302 WB, ICC

Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 104 302 WB, ICC; tested species: rat

Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking.
Larance M, Ramm G, Stöckli J, van Dam EM, Winata S, Wasinger V, Simpson F, Graham M, Junutula JR, Guilhaus M, James DE, et al.
The Journal of biological chemistry (2005) 28045: 37803-13. 104 302 WB

Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release.
Schraw TD, Rutledge TW, Crawford GL, Bernstein AM, Kalen AL, Pessin JE, Whiteheart SW
Blood (2003) 1025: 1716-22. 104 302 WB

A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function.
Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R
The EMBO journal (2000) 1923: 6453-64. 104 302 WB

The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes.
Antonin W, Holroyd C, Tikkanen R, Höning S, Jahn R
Molecular biology of the cell (2000) 1110: 3289-98. 104 302 WB

Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties.
Fasshauer D, Antonin W, Margittai M, Pabst S, Jahn R
The Journal of biological chemistry (1999) 27422: 15440-6. 104 302 WB

Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
Murray RZ, Wylie FG, Khromykh T, Hume DA, Stow JL
The Journal of biological chemistry (2005) 28011: 10478-83. 104 302 IP

SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 104 302 ICC, IHC; KD verified; tested species: mouse

Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein.
Zucchelli E, Pema M, Stornaiuolo A, Piovan C, Scavullo C, Giuliani E, Bossi S, Corna S, Asperti C, Bordignon C, Rizzardi GP, et al.
Molecular therapy. Methods & clinical development (2017) 4: 102-114. 104 302 ICC; tested species: cos cells

Receptor-promoted exocytosis of airway epithelial mucin granules containing a spectrum of adenine nucleotides.
Kreda SM, Seminario-Vidal L, van Heusden CA, O'Neal W, Jones L, Boucher RC, Lazarowski ER
The Journal of physiology (2010) 588Pt 12: 2255-67. 104 302 ICC

Proteomics analysis of insulin secretory granules.
Brunner Y, Couté Y, Iezzi M, Foti M, Fukuda M, Hochstrasser DF, Wollheim CB, Sanchez JC
Molecular & cellular proteomics : MCP (2007) 66: 1007-17. 104 302 ICC

THC Regulates Tearing via Cannabinoid CB1 Receptors.
Thayer A, Murataeva N, Delcroix V, Wager-Miller J, Makarenkova HP, Straiker A
Investigative ophthalmology & visual science (2020) 6110: 48. 104 302 IHC; tested species: mouse

Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.
Demais V, Barthélémy A, Perraut M, Ungerer N, Keime C, Reibel S, Pfrieger FW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3630: 8012-25. 104 302 IHC; tested species: rat

Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo.
Bastow ER, Last K, Golub S, Stow JL, Stanley AC, Fosang AJ
Biology open (2012) 14: 318-28. 104 302 IHC

Cat. No.: 104 302

Amount: 200 µl

Price: $355.00

Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 104 302 WB; tested species: mouse

Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2015) 263: 530-6. 104 302 WB

ICC

IHC-P

Background

Endobrevin/VAMP8, a member of the SNARE family of proteins, is a relative of synaptobrevin that is involved in the fusion of early and late endosomes. Endobrevin is expressed in most mammalian cells but appears to be absent from neurons. The protein is predominantly localized to early and late endosomal membranes but is also found on other membranes and organelles involved in endocytic membrane traffic. In the fusion of late endosomes it forms SNARE complexes with syntaxin 7, syntaxin 8 and vti1b.

1 ) Jahn R et al., Annu. Rev. Biochem. (1999)
2 ) Advani RJ et al., J. Biol. Chem. (1998)
3 ) Wong SH et al., Mol. Biol. Cell (1998)

Protocols

Standard protocol

ICC

IHC

IHC-P

Other products for this target

Endobrevin antibody - 104 302 K.D.

References for Endobrevin - 104 302