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Synaptobrevin2 (VAMP2) antibody - 104 211C5 K.O.

Synaptobrevin2 also known as Vamp2 is a major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 211C5
Amount: 50 µg
Price: $475.00
Cat. No. 104 211C5 50 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 5.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Label Sulfo-Cyanine 5
Clone 69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 104-2P
Data sheet 104_211c5.pdf

References for Synaptobrevin2 - 104 211C5

Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons.
Vevea JD, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4319: 3421-3438. 104 211C5 WB; tested species: rat
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 104 211C5 WB; tested species: mouse
Cat. No.: 104 211C5
Amount: 50 µg
Price: $475.00
Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons.
Vevea JD, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4319: 3421-3438. 104 211C5 WB; tested species: rat
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 104 211C5 WB; tested species: mouse
Background

Synaptobrevins, also known as vesicle-associated membrane proteins (VAMPs), are predominantly expressed in the nervous system and are classified within the brevin subfamily of the SNARE (Soluble NSF Attachment Protein Receptor) protein superfamily. Brevins are small integral transmembrane proteins characterized by a central SNARE motif, an N-terminal cytoplasmic domain, and a C-terminal transmembrane domain. As crucial components of the SNARE machinery, these proteins play an essential role in vesicular transport and membrane fusion processes within cells (1, 2, 3).
In addition to synaptobrevins, the brevin family includes other tissue-specific members such as cellubrevin (VAMP3), myobrevin (VAMP5), and endobrevin (VAMP8), which are expressed in various non-neuronal tissues (4, 5, 6). These isoforms exhibit distinct spatial expression profiles, suggesting specialized functions beyond the nervous system.
Two Synaptobrevin isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their regional distribution within the brain, indicating isoform-specific roles in neuroexocytosis (7).
Synaptobrevin1 (VAMP1) is supposed to be essential for the maintenance of nerve impulse transmission in neuromuscular synapses. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevin2 (VAMP2) is more abundant and widely distributed in the brain and has been shown to be mainly involved in the assembly of effective SNARE complexes, Ca2+-dependent SV exocytosis, and fast endocytosis in hippocampal synapses (8). It is also expressed in spinal cord dorsal horn neurons and implicated in inflammatory pain sensitization (9).
Synaptobrevins are target molecules for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule and thereby disrupt neurotransmitter release (10).