Synaptic Systems - Synaptobrevin2

Cat. No.: 104 211C3

Amount: 50 µg

Price: $470.00

Cat. No. 104 211C3	50 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 1000 gallery IHC: 1 : 200 IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Sulfo-Cyanine 3
Clone	69.1
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity	Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster. No signal: chicken, zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	104-2P
Data sheet	104_211c3.pdf

References for Synaptobrevin2 - 104 211C3

Nanoscale 3D EM reconstructions reveal intrinsic mechanisms of structural diversity of chemical synapses.
Zhu Y, Uytiepo M, Bushong E, Haberl M, Beutter E, Scheiwe F, Zhang W, Chang L, Luu D, Chui B, Ellisman M, et al.
Cell reports (2021) 351: 108953. 104 211C3 IHC; tested species: mouse

Assembly of Excitatory Synapses in the Absence of Glutamatergic Neurotransmission.
Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A
Neuron (2017) 942: 312-321.e3. 104 211C3 IHC; tested species: mouse

Breakdown of axonal synaptic vesicle precursor transport by microglial nitric oxide.
Stagi M, Dittrich PS, Frank N, Iliev AI, Schwille P, Neumann H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 252: 352-62. 104 211C3 IHC

Cat. No.: 104 211C3

Amount: 50 µg

Price: $470.00

ICC

Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Edelmann L et al., EMBO J. (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) McMahon HT et al., Nature (1993)
7 ) Archer BT et al., J. Biol. Chem. (1990)
8 ) Südhof TC et al., Neuron (1989)
9 ) Elferink LA et al., J. Biol. Chem. (1989)
10 ) Baumert M et al., EMBO J. (1989)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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Synaptobrevin2 antibody - 104 211C3 K.O.

References for Synaptobrevin2 - 104 211C3