Cat. No.: 104 211AbRED
Amount: 50 µg
Price:
$465.00
|
|
|
| Cat. No. 104 211AbRED |
50 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR RED.
abberior STAR® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR® dyes established fluorescence detection systems can be used. This product or portions thereof is manufactured under license from abberior GmbH. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: N/A |
| Label | abberior STAR RED |
| Clone | 69.1 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045) |
| Reactivity |
Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster. No signal: chicken, zebrafish. Other species not tested yet. |
| Specificity | K.O. validated |
| Matching control protein/peptide | 104-2P |
| Remarks |
ICC: This antibody conjugate is especially suitable for high resolution STED microscopy. |
| Data sheet | 104_211abred.pdf |
|
|
Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.