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Synaptobrevin2 antibody - 104 211AbOR K.O.

Synaptobrevin2 also known as Vamp2 is a major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 211AbOR
Amount: 50 µg
Price: $465.00
Cat. No. 104 211AbOR 50 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR ORANGE.

abberior STAR® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR® dyes established fluorescence detection systems can be used.

abberior STAR® 635P: λex 638 nm / λem 651 nm
abberior STAR® ORANGE: λex 589 nm / λem 616 nm
abberior STAR® RED: λex 638 nm / λem 655 nm
 

This product or portions thereof is manufactured under license from abberior GmbH.
abberior STAR is a trademark of abberior GmbH, Goettingen/Germany.
Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH.

Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: N/A
Label abberior STAR ORANGE
Clone 69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 104-2P
Remarks

ICC: This antibody conjugate is especially suitable for high resolution STED microscopy.

Data sheet 104_211abor.pdf
Cat. No.: 104 211AbOR
Amount: 50 µg
Price: $465.00
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.