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Synaptobrevin2 (VAMP2) antibody - 104 008 K.O.

Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion
Rabbit monoclonal recombinant IgG
Cat. No.: 104 008
Amount: 50 µg
Price: $420.00
Cat. No. 104 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 AP staining gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 5000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Clone Rb69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 104-2P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Important: Although the epitope is still present in the Botox B cleavage product (aa 1 - 76), it is not recognized, probably due to conformational masking of the epitope.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

Data sheet 104_008.pdf

References for Synaptobrevin2 - 104 008

Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 104 008 WB; tested species: mouse
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 104 008 WB; tested species: mouse
MINSTED nanoscopy enters the Ångström localization range.
Weber M, von der Emde H, Leutenegger M, Gunkel P, Sambandan S, Khan TA, Keller-Findeisen J, Cordes VC, Hell SW
Nature biotechnology (2023) 414: 569-576. 104 008 ICC; tested species: rat
Extracellular vesicle biomarkers for cognitive impairment in Parkinson's disease.
Blommer J, Pitcher T, Mustapic M, Eren E, Yao PJ, Vreones MP, Pucha KA, Dalrymple-Alford J, Shoorangiz R, Meissner WG, Anderson T, et al.
Brain : a journal of neurology (2023) 1461: 195-208. 104 008 ICC; tested species: human
NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic Degradation Maintain Axonal Polarity.
Nabb AT, Bentley M
Molecular biology of the cell (2021) : mbcE21080425. 104 008 ICC; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 104 008 Codex-PC; tested species: human
Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease.
Golec E, Olsson R, Tuysuz EC, Karlsson M, Serjieh Y, King BC, Wennström M, Blom AM
Alzheimer's research & therapy (2025) 171: 11. 104 008 ; tested species: human
Cat. No.: 104 008
Amount: 50 µg
Price: $420.00
Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 104 008 WB; tested species: mouse
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 104 008 WB; tested species: mouse
MINSTED nanoscopy enters the Ångström localization range.
Weber M, von der Emde H, Leutenegger M, Gunkel P, Sambandan S, Khan TA, Keller-Findeisen J, Cordes VC, Hell SW
Nature biotechnology (2023) 414: 569-576. 104 008 ICC; tested species: rat
Extracellular vesicle biomarkers for cognitive impairment in Parkinson's disease.
Blommer J, Pitcher T, Mustapic M, Eren E, Yao PJ, Vreones MP, Pucha KA, Dalrymple-Alford J, Shoorangiz R, Meissner WG, Anderson T, et al.
Brain : a journal of neurology (2023) 1461: 195-208. 104 008 ICC; tested species: human
NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic Degradation Maintain Axonal Polarity.
Nabb AT, Bentley M
Molecular biology of the cell (2021) : mbcE21080425. 104 008 ICC; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 104 008 Codex-PC; tested species: human
Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease.
Golec E, Olsson R, Tuysuz EC, Karlsson M, Serjieh Y, King BC, Wennström M, Blom AM
Alzheimer's research & therapy (2025) 171: 11. 104 008 ; tested species: human
Background

Synaptobrevins, also known as vesicle-associated membrane proteins (VAMPs), are predominantly expressed in the nervous system and are classified within the brevin subfamily of the SNARE (Soluble NSF Attachment Protein Receptor) protein superfamily. Brevins are small integral transmembrane proteins characterized by a central SNARE motif, an N-terminal cytoplasmic domain, and a C-terminal transmembrane domain. As crucial components of the SNARE machinery, these proteins play an essential role in vesicular transport and membrane fusion processes within cells (1, 2, 3).
In addition to synaptobrevins, the brevin family includes other tissue-specific members such as cellubrevin (VAMP3), myobrevin (VAMP5), and endobrevin (VAMP8), which are expressed in various non-neuronal tissues (4, 5, 6). These isoforms exhibit distinct spatial expression profiles, suggesting specialized functions beyond the nervous system.
Two Synaptobrevin isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their regional distribution within the brain, indicating isoform-specific roles in neuroexocytosis (7).
Synaptobrevin1 (VAMP1) is supposed to be essential for the maintenance of nerve impulse transmission in neuromuscular synapses. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevin2 (VAMP2) is more abundant and widely distributed in the brain and has been shown to be mainly involved in the assembly of effective SNARE complexes, Ca2+-dependent SV exocytosis, and fast endocytosis in hippocampal synapses (8). It is also expressed in spinal cord dorsal horn neurons and implicated in inflammatory pain sensitization (9).
Synaptobrevins are target molecules for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule and thereby disrupt neurotransmitter release (10).