Tailor-made Antibodies
and Tools for Life Science

beta-Actin antibody - 251 011

beta-Actin is a major constituent of the cytoskeleton
Mouse monoclonal purified IgG
Cat. No.: 251 011
Amount: 100 µg
Price: $420.00
Cat. No. 251 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Clone 130B4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity Reacts with: rat (P60711), mouse (P60710), zebrafish, human (P60709).
Other species not tested yet.
Specificity May cross-react to α- and γ-actin due to sequence homology.
Remarks

ICC: methanol or PFA fixation

Data sheet 251_011.pdf

References for beta-Actin - 251 011

Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . 251 011 WB; tested species: mouse
Depressed glutamate transporter 1 expression in a mouse model of Dravet syndrome.
Hameed MQ, Hui B, Lin R, MacMullin PC, Pascual-Leone A, Vermudez SAD, Rotenberg A
Annals of clinical and translational neurology (2023) 109: 1695-1699. 251 011 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 251 011 WB; tested species: mouse
PLA2G4E, a candidate gene for resilience in Alzheimer´s disease and a new target for dementia treatment.
Pérez-González M, Mendioroz M, Badesso S, Sucunza D, Roldan M, Espelosín M, Ursua S, Luján R, Cuadrado-Tejedor M, Garcia-Osta A
Progress in neurobiology (2020) 191: 101818. 251 011 WB; tested species: mouse
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 251 011 WB; tested species: rat
Cat. No.: 251 011
Amount: 100 µg
Price: $420.00